Proteomic analysis of Microcystis aeruginosa in response to nitrogen and phosphorus starvation
Proteomic analysis of Microcystis aeruginosa in response to nitrogen and phosphorus starvation
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DOI：
10.1007/s10811-014-0405-4
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发表时间：
2015-06
影响因子：
3.3
通讯作者：
Dongmei Yue;Yuke Peng;Qian Yin;Lin Xiao
中科院分区：
文献类型：
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作者：
Dongmei Yue;Yuke Peng;Qian Yin;Lin Xiao
本文研究了绿藻微囊藻（Microcystis aeruginosa）对氮(N)饥饿、磷(P)饥饿和氮、磷同时饥饿的蛋白质组学和生理反应。缺磷条件下，绿脓杆菌的生长可以维持在接近正常的水平，缺氮条件下，绿脓杆菌的生长明显降低。aeruginosagrowth。橙色类胡萝卜素结合蛋白、金属硫蛋白和几种保守的出口假设蛋白在N饥饿和P饥饿下被发现以相似的方式受到差异调节，这表明这些蛋白在一般的应激反应中起作用。在N饥饿时，参与细胞碳代谢和碳固定的几种蛋白质的表达增强，这可能使它们能够有效地再利用细胞底物并积累糖原以维持长期生存。缺磷时，与蛋白质合成和碳氮同化有关的几种蛋白质下调，表明细胞代谢率普遍降低，从而节省能量和降低功率，维持基础生长。ThoughM。当氮和磷均不存在时，铜绿蛋白的生长显著降低，蛋白质组学组成的显著变化非常有限。
We investigated the proteomic and physiological responses of the bloom-forming cyanobacterium,Microcystis aeruginosa, to nitrogen (N) starvation, phosphorus (P) starvation, and the simultaneous starvation of N and P. Our results suggested thatM. aeruginosacould maintain nearly normal growth under P starvation for at least 7 days, whereas N deficiency significantly decreasedM. aeruginosagrowth. Orange carotenoid-binding proteins, metallothionein, and several conserved exported hypothetical proteins were found differentially regulated with a similar manner responding to N starvation and P starvation, indicating general stress response roles for these proteins. Upon N starvation, the expression of several proteins involved in cellular carbon metabolism and carbon fixation was enhanced, which may enable their effective reuse of cellular substrates and accumulate glycogen for long-term survival. Upon P starvation, several proteins relating to protein synthesis and the assimilation of carbon and N were downregulated, suggesting a general reduction in the cell’s metabolic rate, which could save energy and reducing power to maintain the basal growth. ThoughM. aeruginosagrowth was significantly reduced when both N and P were unavailable, very limited significant changes in the proteomic composition were identified.