TISSUE-SPECIFIC SYNTHESIS OF YOLK PROTEINS IN CAENORHABDITIS-ELEGANS
TISSUE-SPECIFIC SYNTHESIS OF YOLK PROTEINS IN CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0012-1606(83)90322-6
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
SHARROCK, WJ
中科院分区：
文献类型：
--
作者：
KIMBLE, J;SHARROCK, WJ
确定了线虫卵黄蛋白合成的主要部位。在没有性腺细胞(通过激光消融发育早期的性腺前体细胞获得)的动物中，卵黄蛋白含量很高。这表明卵黄蛋白是在性腺外合成的。对解剖分离的组织中存在的蛋白质进行检查，并与体外孵育的分离组织合成的蛋白质进行比较，确定肠道是卵黄蛋白质合成的主要部位。卵黄蛋白可能是在肠道合成的，从肠道分泌到体腔，然后被性腺从体腔吸收到卵母细胞。在4个突变体中也检测了卵黄蛋白合成的位置，这些突变体大部分是雄性体细胞组织，但都是两性生殖系。在这里，卵黄蛋白是由肠道以两性人特有的方式产生的。性别决定可能在肠道组织和生殖系组织中受到协调调控。
The primary site of yolk protein synthesis in the nematode, C. elegans, was determined. In animals containing no gonadal cells (obtained by laser ablation of the gonadal precursor cells early in development), yolk proteins are present in abundance. This demonstrates that yolk proteins are made outside the gonad. An examination of proteins present in tissues isolated by dissection, and a comparison of proteins synthesized by isolated tissues incubated in vitro have identified the intestine as the major site of yolk protein synthesis. Yolk proteins probably are synthesized in the intestine, secreted from the intestine into the body cavity, and taken up from the body cavity by the gonad to reach oocytes. The site of yolk protein synthesis also was examined in 4 mutants that have largely male somatic tissues, but a hermaphrodite germ line. Here again, yolk proteins are produced by intestines in a hermaphrodite-specific manner. Sex determination probably is coordinately regulated in intestinal and germ line tissues.