Comparative expressed-sequence-tag analysis of differential gene expression profiles in BmNPV-infected BmN cells
Comparative expressed-sequence-tag analysis of differential gene expression profiles in BmNPV-infected BmN cells
复制标题
DOI：
10.1006/viro.2001.0823
复制
发表时间：
2001-04-10
期刊：
影响因子：
3.7
通讯作者：
Maeda, S
中科院分区：
文献类型：
--
作者：
Okano, K;Shimada, T;Maeda, S
为了比较未感染和家蚕核多角体病毒（BmNPV）感染的BmN细胞的基因表达谱，我们构建了四个cDNA文库，分别用于模拟感染细胞和感染后2、6和12小时的细胞。利用BLAST检索公共数据库和BmNPV基因组序列，对从文库中获得的2645个est部分序列进行分类。在2、6和12 hp / pi条件下，bmnpv衍生ESTs的比例分别为0.4、4.5和57%。此外，新鉴定出31个BmNPV开放阅读框（orf），其中杆状病毒重复orf （bro）在12个hpi文库中表达水平最高。随着感染的进展，大多数宿主基因的数量减少。然而，有几种，包括细胞色素c氧化酶1，在感染后期增加。两个与细胞凋亡相关的宿主基因也被鉴定出来。(C) 2001学术出版社。
To compare the gene expression profiles of uninfected and Bombyx mori nucleopolyhedrovirus (BmNPV)-infected BmN cells, we constructed four cDNA libraries for mock-infected cells, and cells at 2, 6, and 12 h postinfection (h.p.i.). A total of 2645 partial sequences obtained for expressed-sequence-tags (ESTs) from the libraries were categorized using BLAST searches of the public database and the BmNPV genome sequence. The following proportions of BmNPV-derived ESTs were observed: 0.4, 4.5, and 57% at 2, 6, and 12 h.p.i, respectively. Moreover, 31 BmNPV open reading frames (ORFs) were newly identified for transcripts and the baculovirus-repeated ORFs (bro) showed the highest levels of expression in the 12 h.p.i. library. Most of the host genes decreased in number as the infection progressed. However, several, including cytochrome c oxidase 1, increased in the late stages of infection. Two apoptosis-related host genes were also identified. (C) 2001 Academic Press.