Structural basis of astrocytic Ca2+ signals at tripartite synapses
Structural basis of astrocytic Ca2+ signals at tripartite synapses
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DOI：
10.1038/s41467-020-15648-4
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发表时间：
2020-04-20
影响因子：
16.6
通讯作者：
Nagerl, U. Valentin
中科院分区：
文献类型：
--
作者：
Arizono, Misa;Inavalli, V. V. G. Krishna;Nagerl, U. Valentin
星形胶质细胞Ca 2+信号可以是快速和局部的，支持星形胶质细胞具有调节单个突触的能力的观点。然而，这种特定的信号的解剖学基础仍然不清楚，由于在解决大多数三方突触所在的星形胶质细胞的海绵状结构域的困难。在活体器官型脑切片中使用3D-STED显微镜，我们对星形胶质细胞的海绵状结构域进行成像，并观察到经常形成环状结构的节点和轴的网状网络。这些解剖学特征也在急性海马脑片和在体桶皮质中观察到。大部分树突棘与结节相连，其大小具有相关性。FRAP实验和Ca ~（2+）成像表明，淋巴结是生化区室和Ca ~（2+）微区。将星形胶质细胞Ca 2+信号映射到节点和树突棘的STED图像上，表明它们与单个突触相关。在这里，我们报告的星形胶质细胞的纳米级组织，确定节点作为一个功能性的星形胶质细胞组成的三方突触，可能使神经元和星形胶质细胞之间的突触特异性通信。星形胶质细胞Ca 2+信号可以是快速和局部的，支持星形胶质细胞具有调节单个突触的能力的观点。在这里，作者报告了星形胶质细胞在纳米级水平的组织，并确定节点作为三方突触的功能性星形胶质细胞组成部分。
Astrocytic Ca2+ signals can be fast and local, supporting the idea that astrocytes have the ability to regulate single synapses. However, the anatomical basis of such specific signaling remains unclear, owing to difficulties in resolving the spongiform domain of astrocytes where most tripartite synapses are located. Using 3D-STED microscopy in living organotypic brain slices, we imaged the spongiform domain of astrocytes and observed a reticular meshwork of nodes and shafts that often formed loop-like structures. These anatomical features were also observed in acute hippocampal slices and in barrel cortex in vivo. The majority of dendritic spines were contacted by nodes and their sizes were correlated. FRAP experiments and Ca2+ imaging showed that nodes were biochemical compartments and Ca2+ microdomains. Mapping astrocytic Ca2+ signals onto STED images of nodes and dendritic spines showed they were associated with individual synapses. Here, we report on the nanoscale organization of astrocytes, identifying nodes as a functional astrocytic component of tripartite synapses that may enable synapse-specific communication between neurons and astrocytes. Astrocytic Ca2+ signals can be fast and local, supporting the idea that astrocytes have the ability to regulate single synapses. Here, the authors report the organization of astrocytes at nanoscale level and identify nodes as a functional astrocytic component of tripartite synapses.