Flexible Bioimpedance Sensor for Label-Free Detection of Cell Viability and Biomass
Flexible Bioimpedance Sensor for Label-Free Detection of Cell Viability and Biomass
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DOI：
10.1109/tnb.2015.2451594
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发表时间：
2015-10-01
影响因子：
3.9
通讯作者：
Beskok, Ali
中科院分区：
文献类型：
--
作者：
Fernandez, Renny Edwin;Lebiga, Elise;Beskok, Ali
我们介绍了一种灵活的微流控生物阻抗传感器，它能够检测细胞悬浮液中生物量和细胞活力的变化。该传感器是在镀铟锡氧化物(ITO)的聚对苯二甲酸乙二醇酯(PET)衬底上开发的，不含金、硅、PDMS或玻璃。与定制的印刷电路板读出模块相结合，细胞悬浮液的阻抗特性可以在样品引入后一分钟内测量，液体体积小于5 mU L。便携式传感器系统占用的工作台空间非常小，有可能被开发为一次性生物阻抗探头，用于快速检测生物悬浮液中的介电变化。该传感器设计用于产生具有双电极对系统的细胞悬浮液所特有的微分阻抗谱。在这项研究中，该传感器具有区分活的和热处理的酿酒酵母的潜力。这种一次性传感器以及距离变化技术被吹捧为现有的一些在线一次性生物量检测探头和电化学传感器的廉价替代品。
We introduce a flexible microfluidic bioimpedance sensor that is capable of detecting biomass and cell viability variations in a cell suspension. The sensor is developed on indium tin oxide (ITO) coated polyethylene terephthalate (PET) substrate and is devoid of gold, silicon, PDMS, or glass. In conjugation with a custom built PCB read-out module, the impedance characteristics of a cell suspension can be measured within one minute of sample introduction using liquid volumes less than 5 mu L. The portable sensor system occupies very little bench space and has the potential to be developed as a disposable electrical bioimpedance probe for rapid detection of dielectric variations in a biological suspension. The sensor is designed to generate a differential impedance spectra exclusive to a cell suspension with a dual-electrode-pair system. The potential of the sensor to discriminate between live and heat treated Saccharomyces cerevisiae is demonstrated in this study. The disposable sensor along with the distance variation technique is touted to be an inexpensive alternative to some of the existing online disposable biomass detection probes and electrochemical sensors.