Formation of acyl and alkenyl glycerol derivatives in Clostridium butyricum.
Formation of acyl and alkenyl glycerol derivatives in Clostridium butyricum.
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DOI：
10.1016/0005-2760(70)90239-0
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发表时间：
1970-02-10
期刊：
影响因子：
--
通讯作者：
Lands, W E
中科院分区：
文献类型：
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作者：
Hill, E E;Lands, W E
缩醛磷脂的合成机制目前尚不清楚，但已描述了完整的系统1-B将标记的磷酸盐、乙酸盐、脂肪酸和脂肪醛掺入缩醛磷脂中。此类同位素研究提出了多种导致缩醛磷脂合成的途径。这些提出的途径包括烷基醚键的脱氢、酰基酯键的还原以及脂肪醛和甘油缩合形成的半缩醛的脱水。在本研究中，我们观察到标记甘油掺入丁酸梭菌的二酰基磷酸甘油酯和烯基酰基磷酸甘油酯中。丁酸 (ATCC 6015) 在 37°C 的培养基中厌氧生长，如 WOLFE AND O’KANE~ 所描述，含有添加的盐并使用碱性连苯三酚塞 8 来维持厌氧条件。在对数中期至晚期通过离心收集细胞，用0.03 M磷酸钠（pH 7.0）洗涤并在标记甘油存在下重悬于新鲜培养基中。在 37” 下孵育后，按照 GOLDFINE 和 BLOCH~ 的描述制备脂质提取物。
The mechanism of synthesis of plasmalogens is not currently known, but the incorporation of labeled phosphate, acetate, fatty acids, and fatty aldehydes into plasmalogens has been described for intact systemsl-B. Such isotopic studies have suggested a variety of pathways leading to the synthesis of plasmalogens. Among these proposed pathways are the dehydrogenation of the alkyl ether bond, the reduction of an acyl ester bond, and the dehydration of the hemiacetal formed by the condensation of a fatty aldehyde and glycerol. In the present study, we have observed the incorporation of labeled glycerol into diacyl phosphoglycerides and alkenyl acyl phosphoglycerides of Clostridium butyricum.C. butyricum (ATCC 6015) was grown anaerobically at 37O in a medium, described by WOLFE AND O’KANE~, containing added salts and using alkaline pyrogallol plugs8 to maintain anaerobic conditions. Cells were collected by centrifugation during mid to late log phase, washed with 0.03 M sodium phosphate (pH 7.0) and resuspended in fresh medium in the presence of labeled glycerol. After incubation at 37”, lipid extracts were prepared as described by GOLDFINE AND BLOCH~.