Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria
Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria
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DOI：
10.1007/978.1.60761.925.3_5
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Koike, Hiroyuki
中科院分区：
文献类型：
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作者：
Shen, Jian-Ren;Kawakami, Keisuke;Koike, Hiroyuki
本章描述了从嗜热蓝细菌Thermosynechococcus vulcanus中纯化和结晶放氧光系统II核心二聚体复合物。从蓝细菌中纯化光系统II的方法包括培养细胞，分离类囊体膜，通过去污剂增溶纯化粗制和纯的光系统II核心复合物，随后进行差速离心和柱层析。纯化的核心二聚体颗粒成功地用于结晶，这些纯化和结晶程序可用于另一种嗜热的细长蓝细菌
This chapter describes the purification and crystallization of oxygen evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T elongatus