Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion
Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion
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DOI：
10.1073/pnas.0907549107
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发表时间：
2010-01-12
影响因子：
11.1
通讯作者：
Cervone, Felice
中科院分区：
文献类型：
--
作者：
Lionetti, Vincenzo;Francocci, Fedra;Cervone, Felice
植物细胞壁代表了生物燃料和其他有用产品的丰富的可再生来源。将其转化为单糖（糖化）并随后通过微生物转化为乙醇或其他产品的工业规模化的主要瓶颈是它们对酶促糖化的不耐受。我们研究了嵌入纤维素-半纤维素网络的果胶结构是否影响纤维素对酶的暴露，从而影响糖化过程。通过表达真菌多聚半乳糖醛酸酶（PG）或果胶甲酯酶抑制剂（PMEI），减少拟南芥植物中去甲基酯化的同型半乳糖醛酸（HGA），从而提高酶促糖化的效率。在转化植物中观察到的酶糖化效率的提高也可以减少酸预处理的需要。在表达PG的烟草植物和表达PMEI的小麦植物中获得了类似的结果，表明去甲基酯化的HGA的还原可用于作物物种以促进生物质糖化过程。
Plant cell walls represent an abundant, renewable source of biofuel and other useful products. The major bottleneck for the industrial scale-up of their conversion to simple sugars (saccharification), to be subsequently converted by microorganisms into ethanol or other products, is their recalcitrance to enzymatic saccharification. We investigated whether the structure of pectin that embeds the cellulose-hemicellulose network affects the exposure of cellulose to enzymes and consequently the process of saccharification. Reduction of de-methyl-esterified homogalacturonan (HGA) in Arabidopsis plants through the expression of a fungal polygalacturonase (PG) or an inhibitor of pectin methylesterase (PMEI) increased the efficiency of enzymatic saccharification. The improved enzymatic saccharification efficiency observed in transformed plants could also reduce the need for acid pretreatment. Similar results were obtained in PG-expressing tobacco plants and in PMEI-expressing wheat plants, indicating that reduction of de-methyl-esterified HGA may be used in crop species to facilitate the process of biomass saccharification.