Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection
Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection
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DOI：
10.1046/j.1365-2583.2000.00162.x
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发表时间：
2000-02-01
影响因子：
2.6
通讯作者：
Beaty, BJ
中科院分区：
文献类型：
--
作者：
Olson, KE;Myles, KM;Beaty, BJ
构建了双亚基因组辛德毕斯病毒（dsSIN）MRE/3 '2 J/GFP，经口感染埃及伊蚊后，在中肠中高效表达绿色荧光蛋白（GFP）。MRE/3 '2 J/GFP RNA基因组包含dsSIN病毒TE/3' 2 J/GFP的非结构基因和顺式作用序列，但具有MRE 16 SIN病毒的结构基因。与TE/3 '2 J/GFP病毒不同，MRE/3' 2 J/GFP病毒有效地经口感染蚊子。在感染后1-2天，观察到GFP在中肠的内腔侧上作为多个表达灶。在感染后10-12天，15只感染MRE/3 '2 J/GFP病毒的蚊子中有13只在蚊子中肠中具有高水平的GFP表达。MRE 3 ′ 2 J dsSIN表达系统是一种在Ae.埃及中肠
A double subgenomic Sindbis (dsSIN) virus, MRE/3'2 J/GFP, was constructed to efficiently express green fluorescent protein (GFP) in the midgut of Aedes aegypti following per os infection. The MRE/3'2 J/GFP RNA genome contained the nonstructural genes and cis-acting sequences of the dsSIN virus, TE/3'2 J/GFP, but had the structural genes of MRE16 SIN virus. MRE/3'2 J/GFP virus, unlike TE/3'2 J/GFP virus, efficiently infected mosquitoes orally. At 1-2 days postinfection, GFP was observed as multiple foci of expression on the lumenal side of the midgut. At 10-12 days postinfection, thirteen of fifteen mosquitoes infected with MRE/3'2 J/GFP virus had high levels of GFP expression in the mosquito midgut. The MRE3'2 J dsSIN expression system should be an important tool for efficient gene expression in Ae. aegypti midguts.