Sperm maturation, migration, and localization before and after copulation in black rockfish (Sebastes schlegelii)
Sperm maturation, migration, and localization before and after copulation in black rockfish (Sebastes schlegelii)
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黑石斑鱼（Sebastes schlegelii）交配前后精子的成熟、迁移和定位
DOI：
10.1016/j.theriogenology.2021.01.001
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发表时间：
2021-03-09
期刊：
影响因子：
2.8
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Zhao, Haixia;Wang, Xueying;Li, Jun
施氏硬骨鱼是一种典型的胎生性硬骨鱼，精子储存期为6个月，从11月到4月。本研究分别用电子显微镜、计算机辅助精子分析仪和组织学方法研究了精子的形态、生理特性和精子在雌性卵巢中的定位。在交配过程中，我们观察到精巢内的精子具有成熟的结构，头部呈杆状，中段较短，鞭毛较长。进一步证实精子在卵巢液中高速游动。交配后，仅在保存初期(11~12月)的卵巢液中可见游动精子，大部分精子随机散布在卵巢腔内，部分集中在卵母细胞与卵泡柄之间的隐窝内。此后，卵母细胞周围的卵巢上皮迅速增殖，将精子包裹在卵泡层外，并在卵泡层外形成大量隐窝，作为精子的储存场所，直到受精。这一发现将有助于进一步了解胎生性硬骨鱼精子长期储存的机制。？2021由爱思唯尔公司出版。
Sebastes schlegelii is a typical viviparous teleost with six months sperm storage duration from November to April. In this study, spermatozoa morphological and physiological characteristics and sperm location in the female ovary were investigated by electron microscopy, computer-assisted sperm analyzer and histologic analysis, respectively. During copulation, we observed that spermatozoa in the testis had mature structure with rod-shaped head, a short midpiece, and a long flagellum. And further verified sperm swam freely at a high speed in the ovary fluid. After copulation, we only found swimming sperm in the ovary fluid at the early storage stage (November to December) and the majority of sperm were scattered randomly in the ovary cavity and partially concentrated in the crypt between the oocyte and stalk of follicle. Thereafter, the ovarian epithelium around the oocytes proliferated rapidly and wrapping spermatozoa outside of the follicular layer and formed a lot of crypts outside of the follicular layer which served as the sperm storage site until fertilization. The present findings would be useful for further understanding the mechanism of long-term sperm storage in viviparous teleost. ? 2021 Published by Elsevier Inc.