A streamlined protocol for emulsion polymerase chain reaction and subsequent purification
A streamlined protocol for emulsion polymerase chain reaction and subsequent purification
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DOI：
10.1016/j.ab.2010.11.029
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发表时间：
2011-03-01
影响因子：
2.9
通讯作者：
Gloekler, Joern
中科院分区：
文献类型：
--
作者：
Schuetze, Tatjana;Rubelt, Florian;Gloekler, Joern
Compartmentalization of polymerase chain reaction (PCR) reduces artifacts, especially when complex libraries are amplified. It allows clonal amplification of templates from complex mixtures in a bias-free manner. Here we describe a rapid, straightforward, and easy protocol for PCR in a water-in-oil emulsion (ePCR) including sample recovery by DNA purification. Furthermore, no special laboratory equipment is needed and inexpensive components are used. Therefore, our flexible protocol allows ePCR to be readily implemented in daily routine experiments for a broad range of applications. (C) 2010 Elsevier Inc. All rights reserved.
