Limiting dilution analysis of the stem cells for T cell lineage.
Limiting dilution analysis of the stem cells for T cell lineage.
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T 细胞谱系干细胞的有限稀释分析。
DOI：
10.4049/jimmunol.137.8.2434
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发表时间：
1986
影响因子：
4.4
通讯作者：
S. Nishikawa
中科院分区：
文献类型：
--
作者：
Y. Katsura;T. Kina;T. Amagai;T. Tsubata;K. Hirayoshi;Y. Takaoki;T. Sado;S. Nishikawa
通过静脉或胸腺内（i.t.）移植，比较研究了骨髓、脾、胸腺和胎肝细胞的T细胞系干细胞活性。途径进入右腿和尾部屏蔽（L-T屏蔽）和900 R辐射受体小鼠，这些小鼠能够在不提供造血干细胞的情况下存活。将来自B10.Thy-1.1（H-2b，Thy-1.1）小鼠的细胞连续稀释并转移到L-T屏蔽和辐射的C57 BL/6（H-2b，Thy-1.2）小鼠中，21天后通过流式细胞荧光法测定受体小鼠胸腺细胞的Thy-1.1+细胞。将具有供体型T细胞的受体小鼠的百分比相对于转移的细胞数作图，并且将每种细胞来源中的干细胞活性表示为50%阳性值，即在50%受体小鼠的胸腺中产生供体型T细胞所需的供体细胞数。在i. v.转移实验中，骨髓细胞的活性与胎肝细胞相似，分别比脾细胞和胸腺细胞高约100倍和近1000倍。在信息技术领域。在i. v.转移实验中，产生供体型T细胞所需的细胞数量远低于i. v.转移实验，尽管i. v.和i.t.细胞来源之间的转移不同。在信息技术领域。骨髓细胞的50%阳性率分别是胎肝细胞、脾细胞和胸腺细胞的5倍、400倍和500倍。我们先前的发现，即干细胞富集在7天前全身照射和骨髓重建的小鼠的脾脏中，这也通过在i. v.以及i.t.中进行的本有限稀释测定得到证实。转账。
Stem cell activities of bone marrow, spleen, thymus, and fetal liver cells for T cell lineage were studied comparatively by transferring the cells from these organs through i.v. or intrathymus (i.t.) route into right leg- and tail-shielded (L-T-shielded) and 900 R-irradiated recipient mice, which were able to survive without supplying hemopoietic stem cells. Cells from B10.Thy-1.1 (H-2b, Thy-1.1) mice were serially diluted and were transferred into L-T-shielded and irradiated C57BL/6 (H-2b, Thy-1.2) mice, and 21 days later the thymus cells of recipient mice were assayed for Thy-1.1+ cells by flow cytofluorometry. The percentage of recipient mice possessing donor-type T cells was plotted against the number of cells transferred, and the stem cell activity in each cell source was expressed as the 50% positive value, the number of donor cells required for generating donor-type T cells in the thymuses of 50% of recipient mice. In i.v. transfer experiments, the activity of bone marrow cells was similar to that of fetal liver cells, and about 100 times and nearly 1000 times higher than those of spleen cells and thymus cells, respectively. In i.t. transfer experiments, the number of cells required for generating donor-type T cells was much lower than that in i.v. transfer experiments, although the ratio in 50% positive values between i.v. and i.t. transfers differed among cell sources. In i.t. transfers, the 50% positive value of bone marrow cells was five times, 400 times, and 500 times higher than that of fetal liver cells, spleen cells, and thymus cells, respectively. Our previous finding that stem cells are enriched in the spleens of mice which were whole body-irradiated and marrow-reconstituted 7 days earlier was confirmed also by the present limiting dilution assay carried out in i.v. as well as i.t. transfers.