Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins
Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins
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DOI：
10.1016/s0092-8674(00)81047-8
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发表时间：
1996-03-08
期刊：
影响因子：
64.5
通讯作者：
Nasmyth, K
中科院分区：
文献类型：
--
作者：
Jansen, RP;Dowzer, C;Nasmyth, K
某些细胞类型产生的后代在其环境中没有任何差异的情况下采用不同的基因表达模式。芽殖酵母的细胞产生母细胞和子细胞，它们的不同之处在于只有母细胞表达HO核酸内切酶基因，从而转换交配类型。我们描述了五个基因的鉴定，称为SHE 1-SHE 5，编码母亲特异性HO表达所需的细胞质蛋白。She 1 p与微小肌球蛋白Myo 4p相同，但She 3 p不是母亲特异性蛋白。相反，它们在生长的芽中积累。她的蛋白质可能需要运输的因素，促进HO抑制从母细胞进入其芽。在随附的论文中，我们表明，SHE基因是必要的积累在女儿的细胞核的Ash 1 p，一个阻遏HO。
Certain cell types give rise to progeny that adopt different patterns of gene expression in the absence of any differences in their environment. Cells of budding yeast give birth to mother and daughter cells that differ in that only mother cells express the HO endonuclease gene and thereby switch mating types. We describe the identification of five genes, called SHE1-SHE5, that encode cytoplasmic proteins required for mother-specific HO expression. She1p, which is identical to the minimyosin Myo4p, and She3p are not, however, mother-specific proteins. On the contrary, they accumulate in growing buds. She proteins might be required for the transport of factors that promote HO repression from the mother cell into its bud. In an accompanying paper, we show that SHE genes are needed for the accumulation in daughter nuclei of Ash1p, a repressor of HO.