CELLULAR RECEPTOR FOR POLIOVIRUS - MOLECULAR-CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF A NEW MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY
CELLULAR RECEPTOR FOR POLIOVIRUS - MOLECULAR-CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF A NEW MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY
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DOI：
10.1016/0092-8674(89)90690-9
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发表时间：
1989-03-10
期刊：
影响因子：
64.5
通讯作者：
RACANIELLO, VR
中科院分区：
文献类型：
--
作者：
MENDELSOHN, CL;WIMMER, E;RACANIELLO, VR
对感染灵长类动物宿主中一些部位的脊髓灰质炎病毒复制的限制似乎受到病毒受体的表达控制。为了更多地了解这些结合位点及其在病毒组织营养不良中的作用，分离了编码功能性脊髓灰质炎病毒受体的cDNA克隆。预测的氨基酸序列表明，人脊髓灰质炎病毒受体是一种积分的膜蛋白，具有保守的氨基酸和域结构的特征，是免疫球蛋白超家族成员的特征。北部杂交分析表明，与病毒结合位点的有限表达相反，脊髓灰质炎病毒受体转录物在广泛的人体组织中表达，这表明需要对受体蛋白的其他因素或修饰来允许脊髓灰质炎病毒的附着。
Restriction of poliovirus replication to a few sites in the infected primate host appears to be controlled by the expression of viral receptors. To learn more about these binding sites and their role in viral tissue trophism, cDNA clones encoding functional poliovirus receptors were isolated. The predicted amino acid sequence reveals that the human poliovirus receptor is an integral membrane protein with the conserved amino acids and domain structure characteristic of members of the immunoglobulin superfamily. Northern hybridization analysis indicates that poliovirus receptor transcripts are expressed in a wide range of human tissues, in contrast to the limited expression of virus binding sites, which suggests that additional factors or modifications of the receptor protein are required to permit poliovirus attachment.