THE WHITE GENE AS A MARKER IN A NEW P-ELEMENT VECTOR FOR GENE-TRANSFER IN DROSOPHILA
THE WHITE GENE AS A MARKER IN A NEW P-ELEMENT VECTOR FOR GENE-TRANSFER IN DROSOPHILA
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DOI：
10.1093/nar/15.10.3947
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发表时间：
1987-05-26
影响因子：
14.9
通讯作者：
GEHRING, WJ
中科院分区：
文献类型：
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作者：
KLEMENZ, R;WEBER, U;GEHRING, WJ
我们描述了新的载体，适用于P-元件介导的种系转化ofDrosophila melanogaster使用乘客基因，其表达不会导致一个容易检测到的表型变化的转化苍蝇。P元件载体含有与热休克蛋白70（hsp 70）基因启动子融合的白色基因。即使没有热处理，白色基因的表达也部分或完全挽救了受体果蝇的白色表型。大多数创始动物（GO）的转化后代分为两类，它们的橙子和红色眼睛是可以区分的。不同水平的白色表达可能是由于与不同染色体插入位点相关的位置效应。橙子眼睛果蝇种群中基因剂量加倍导致眼睛颜色明显变暗。因此，由于纯合转化体和杂合转化体的表型差异，通过简单的近交可以容易地产生纯合转化体。通过存在具有8和12个独特限制性位点的多接头促进克隆到这些P元件载体中。
We describe new vectors suitable for P-element mediated germ line transformation ofDrosophila melanogasterusing passenger genes whose expression does not result in a readily detectable phenotypic change of the transformed flies. The P-element vectors contain the white gene fused to the heat shock protein 70 (hsp70) gene promoter. Expression of the white gene rescues the white phenotype of recipient flies partly or completely even without heat treatment. Transformed descendents of most founder animals (GO) fall into two classes which are distinguishable by their orange and red eye colours. The different levels of white expression are presumably due to position effects associated with different chromosomal sites of insertion. Doubling of the gene dose in orange eyed fly stocks results in an easily visible darkening of the eye colour. Consequently, the generation of homozygous transformants is easily possible by simple Inbreeding due to the phenotypic distinction of homo- and heterozygous transformants. Cloning into these P-element vectors is facilitated by the presence of polylinkers with 8 and 12 unique restriction sites.