MODE OF INHIBITION OF DIPHTHERIA TOXIN BY AMMONIUM CHLORIDE
MODE OF INHIBITION OF DIPHTHERIA TOXIN BY AMMONIUM CHLORIDE
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DOI：
10.1128/jb.90.6.1557-1562.1965
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发表时间：
1965-01-01
影响因子：
3.2
通讯作者：
GROMAN, NB
中科院分区：
文献类型：
--
作者：
KIM, K;GROMAN, NB
铵盐对白喉毒素的抑制作用在100倍的毒素范围内与毒素浓度无关。通过降低 pH 值可消除最低浓度氯化铵的抑制作用，表明游离氨是抑制剂的活性形式。单次添加氯化铵可以在有限的时间内抑制毒素，但在没有抗毒素的情况下，需要定期重新添加铵盐才能无限期地维持抑制作用。毒素没有被破坏，并且在存在或不存在氯化铵的情况下其吸附效果均相同。添加氯化铵也能有效抑制预吸附毒素。被抑制的毒素仍可被抗毒素中和。通过添加琥珀酸或还原型烟酰胺腺嘌呤二核苷酸来逆转氨抑制的尝试未成功。通过干扰主动运输来抑制毒素的尝试也没有成功。
The inhibition of diphtheria toxin by ammonium salts was independent of toxin concentration over a 100-fold range of toxin. Inhibition by minimal concentrations of ammonium chloride was abolished by lowering the pH, indicating that free ammonia is the active form of inhibitor. A single addition of ammonium chloride inhibited toxin for a limited period of time, but periodic readdition of the ammonium salt was required to sustain inhibition indefinitely in the absence of antitoxin. Toxin was not destroyed and its adsorption occurred equally well in the presence or absence of ammonium chloride. Preadsorbed toxin was also effectively inhibited by the addition of ammonium chloride. Inhibited toxin remained accessible to antitoxin neutralization. Attempts to reverse ammonia inhibition by the addition of succinate or reduced nicotinamide adenine dinucleotide were unsuccessful. Attempts to inhibit toxin by interfering with active transport were also unsuccessful.