FLUORESCAMINE - REAGENT FOR ASSAY OF AMINO-ACIDS, PEPTIDES, PROTEINS, AND PRIMARY AMINES IN PICOMOLE RANGE

FLUORESCAMINE - REAGENT FOR ASSAY OF AMINO-ACIDS, PEPTIDES, PROTEINS, AND PRIMARY AMINES IN PICOMOLE RANGE
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DOI:
10.1126/science.178.4063.871
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发表时间:
1972-01-01
期刊:
影响因子:
56.9
通讯作者:
DAIRMAN, W
DAIRMAN, W
中科院分区:
综合性期刊1区
文献类型:
--
作者:
UDENFRIEND, S;STEIN, S;DAIRMAN, W

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荧光胺是一种检测皮摩尔范围伯胺的新试剂。在室温下,它与胺的反应几乎是瞬间的。产物是高荧光的,而试剂及其降解产物是非荧光的。讨论了应用。
Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent. Applications are discussed.