Confirmation by LC-MS of drugs in oral fluid obtained from roadside testing
Confirmation by LC-MS of drugs in oral fluid obtained from roadside testing
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DOI：
10.1016/j.forsciint.2007.03.034
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发表时间：
2007-08-06
影响因子：
2.2
通讯作者：
Lopez-Rivadulla, Manuel
中科院分区：
文献类型：
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作者：
Concheiro, Marta;de Castro, Ana;Lopez-Rivadulla, Manuel
本研究的目的是评估两个目前的现场口服液（OF）药物检测设备（OraLab和Drager）的有效性，作为西班牙参与路边测试评估项目（ROSITA项目）的一部分。这项研究是2004年和2005年在加利西亚（西班牙西北部）与西班牙交通警察合作进行的。共有468名司机在警察控制中选择同意通过知情同意参与。此外，还收集了唾液样本，并送往实验室确认现场结果。为此，使用两种不同的分析液相色谱-质谱法（LC-MS）方法检测300 μ L样品中的II类药物或代谢物。吗啡，6-乙酰吗啡，安非他明，甲基安非他明，MDA，MDMA，MDEA，MBDB，可卡因和苯甲酰芽子碱的同时分析进行使用100 μ L的口服液，自动固相萃取后。采用不同的LC-MS方法，在pH 6下用己烷进行液-液萃取，以检测200 μ L口服液中的Δ（9）-THC。两种方法均经过充分验证，包括线性（1-250 ng/mL，2-250 ng/mL）回收率（>50%）、日内和日间精密度（CV < 15%）、准确度（平均相对误差< 15%）、检测限（0.5和1 ng/mL）、定量（1和2 ng/mL）和基质效应。对所有阳性病例和随机选择的30%阴性病例进行确认分析。OraLab对可卡因和阿片类药物的敏感性、特异性、准确性、阳性预测值和阴性预测值均> 90%，Drager对可卡因的敏感性、特异性、准确性、阳性预测值和阴性预测值均> 90%。然而，对于两种检测装置，其他化合物的结果可以得到改善。观察到易用性和解释模式（目视或仪器）的差异。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
The aim of this study was to assess the effectiveness of two current on-site oral fluid (OF) drug detection devices (OraLab and Drager), as part of the Spanish participation in the Roadside Testing Assessment Project (ROSITA Project). The study was done in collaboration with the Spanish Traffic Police, in Galicia (NW Spain), during 2004 and 2005. A total of 468 drivers selected at the police controls agreed to participate through informed consent. In addition, saliva samples were collected-and sent to the laboratory to confirm the on-site results. For this purpose, two different analytical liquid chromatography-mass spectrometry (LC-MS) methods were used to detect I I drugs or metabolites in a 300 mu L sample. Simultaneous analysis of morphine, 6-acetylmorphine, amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB, cocaine and benzoylecgonine was carried out using 100 mu L of oral fluid, after an automated solid phase extraction. A different LC-MS method was performed to detect Delta(9)-THC in 200 mu L of oral fluid using liquid-liquid extraction with hexane at pH 6. Both methods were fully validated, including linearity (1-250 ng/mL, 2-250 ng/mL) recovery (>50%), within-day and between-day precision (CV < 15%), accuracy (mean relative error < 15%), limit of detection (0.5 and I ng/mL), quantitation (I and 2 ng/mL) and matrix effect. All of the positive cases and a random selection of 30% of the negatives were analyzed for confirmation analysis. Good results (sensitivity, specificity, accuracy, positive predictive value and negative predictive value > 90%) were obtained for cocaine and opiates by OraLab, and for cocaine by Drager. However, the results for the other compounds could be improved for both detection devices. Differences in the ease of use and in the interpretation mode (visual or instrumental) were observed. (c) 2007 Elsevier Ireland Ltd. All rights reserved.