Microwave-Assisted Synthesis of GlycopolymerFunctionalized Silver Nanoclusters: Combining the Bioactivity of Sugar with the Fluorescence and Cytotoxicity of Silver
Microwave-Assisted Synthesis of GlycopolymerFunctionalized Silver Nanoclusters: Combining the Bioactivity of Sugar with the Fluorescence and Cytotoxicity of Silver
复制标题
微波辅助合成糖聚合物功能化银纳米团簇：将糖的生物活性与银的荧光和细胞毒性相结合
DOI：
10.1002/marc.201300905
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发表时间：
2014
影响因子：
4.6
通讯作者：
Chen Gaojian
中科院分区：
文献类型：
--
作者：
Lu Wei;Ma Wenjuan;Lu Jiawei;Li Xiao;Zhao Yun;Chen Gaojian
通过RAFT聚合合成2-(甲基丙烯酰氨基)吡喃葡萄糖(MAG)和甲基丙烯酸(MAA)的共聚物，然后以微波辐射为模板制备糖聚合物功能化的银纳米团簇(Gly-Ag NCs)。通过 NMR、GPC、UV-Vis、SEM、TEM、AAS 和荧光光谱对聚合物和所得纳米团簇进行表征。使用 GLUT-1 过表达癌细胞 K562 进一步检查荧光 Gly-Ag NC 的生物活性。 Gly-Ag NC 对 K562 细胞表现出有效的结合能力，并以剂量依赖性方式抑制细胞活力 (IC50 = 0.65 μg mL–1)，表明它们在癌症成像和靶向癌症治疗方面具有潜在的生物学应用。
Copolymers of 2‐(methacrylamido)glucopyranose (MAG) and methacrylic acid (MAA) are synthesized by RAFT polymerization and then used as templates to prepare glycopolymer‐functionalized Ag nanoclusters (Gly‐Ag NCs) through microwave irradiation. Polymers and the resulting nanoclusters are characterized by NMR, GPC, UV‐Vis, SEM, TEM, AAS and fluorescence spectroscopy. The bio‐activity of the fluorescent Gly‐Ag NCs are further examined using GLUT‐1 over‐expressing cancer cells K562. Gly‐Ag NCs show efficient binding ability toward K562 cells and inhibit the cell viability in a dose dependent manner (IC50 = 0.65 μg mL–1), indicating their potential biological applications for both cancer imaging and targeted cancer therapy.