Stresses at the cell-to-substrate interface during locomotion of fibroblasts
Stresses at the cell-to-substrate interface during locomotion of fibroblasts
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DOI：
10.1016/s0006-3495(99)77386-8
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发表时间：
1999-04-01
影响因子：
3.4
通讯作者：
Wang, YL
中科院分区：
文献类型：
--
作者：
Dembo, M;Wang, YL
最近的技术改进的弹性基板的方法，使它有可能产生空间分辨测量的牵引力所施加的单个运动细胞。在这项研究中，我们已经应用这些发展，以产生3 T3成纤维细胞在稳定运动过程中施加的牵引力的地图。所得图像具有类似于5 μ m的空间分辨率和类似于10（2）kdyn/cm（2）（10（4）pN/μ m（2））的最大强度。我们发现，成纤维细胞运动的推进推力，类似于0.2达因，被赋予到15 μ m的前缘内的底层。这些观察结果表明，成纤维细胞的板状伪足能够产生强烈的牵引应力。细胞体和后部似乎是机械被动的结构，完全被这个动作向前拉动。
Recent technological improvements in the elastic substrate method make it possible to produce spatially resolved measurements of the tractions exerted by single motile cells. In this study we have applied these developments to produce maps of the tractions exerted by 3T3 fibroblasts during steady locomotion. The resulting images have a spatial resolution of similar to 5 mu m and a maximum intensity of similar to 10(2) kdyn/cm(2) (10(4) pN/mu m(2)). We find that the propulsive thrust for fibroblast locomotion, similar to 0.2 dyn, is imparted to the substratum within 15 mu m of the leading edge. These observations demonstrate that the lamellipodium of the fibroblast is able to generate intense traction stress. The cell body and posterior seem to be mechanically passive structures pulled forward entirely by this action.