Changes in nitric oxide and inducible nitric oxide synthase following stretch-induced injury to the tibialis anterior muscle of rabbit.
Changes in nitric oxide and inducible nitric oxide synthase following stretch-induced injury to the tibialis anterior muscle of rabbit.
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DOI：
10.2170/jjphysiol.r614
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发表时间：
2005-04
期刊：
影响因子：
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通讯作者：
T. Sakurai;J. Hollander;S. Brickson;H. Ohno;L. Ji;T. Izawa;T. Best
中科院分区：
文献类型：
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作者：
T. Sakurai;J. Hollander;S. Brickson;H. Ohno;L. Ji;T. Izawa;T. Best
本研究观察了急性肌肉牵张损伤后0、4、12、24和48 h一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）含量和酶活性的变化。对30只雄性新西兰白色兔（每个时间点n = 6）的胫骨前肌诱导单次牵拉损伤。收集损伤和未损伤的对侧假手术肌肉，并在每个时间点分析NO水平、iNOS含量和iNOS活性。此外，使用三只动物来估计基线NO水平和iNOS活性。至术后24 h和牵拉损伤后，损伤肌肉和假手术肌肉中NO含量逐渐降低（p < 0.05）。伤后48h，伤后肌肉NO含量比假手术组高146%（p < 0.05）。伤后4h和48h iNOS蛋白含量显著高于假手术组（p < 0.05）。同样，损伤肌肉中iNOS活性在4小时（p < 0.05）和48小时（p < 0.01）高于假手术肌肉。这些结果表明，NO可能在骨骼肌损伤后的恢复过程中发挥积极作用，通过iNOS的表达调节。
This study investigated the changes in nitric oxide (NO) together with inducible nitric oxide synthase (iNOS) content and enzyme activity at 0, 4, 12, 24, and 48 h following acute muscle stretch injury. A single stretch injury was induced to the tibialis anterior muscle of 30 male New Zealand white rabbits (n = 6 at each time point). Injured and uninjured contralateral sham-operated muscles were harvested and analyzed for NO levels, iNOS content, and iNOS activity at each time point. Furthermore, three animals were used to estimate baseline NO levels and iNOS activity. There was a progressive reduction in NO content in the injured and the sham-operated muscles up to 24 h postoperation and stretch injury (p < 0.05). At 48 h postinjury, however, NO levels were 146% higher in injured muscles than in sham-operated muscles (p < 0.05). iNOS protein content was higher at 4 h and 48 h in injured versus shamoperated muscles (p < 0.05). Similarly, iNOS activity was higher at 4 h (p < 0.05) and at 48 h (p < 0.01) in injured versus sham-operated muscles. These results suggest that NO may play an active role during the postinjury recovery of skeletal muscle modulated by iNOS expression.