Suitability and perspectives on using recombinant insect cells for the production of virus-like particles
Suitability and perspectives on using recombinant insect cells for the production of virus-like particles
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DOI：
10.1007/s00253-013-5474-9
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发表时间：
2014-01
影响因子：
5
通讯作者：
H. Yamaji
中科院分区：
文献类型：
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作者：
H. Yamaji
病毒样颗粒 (VLP) 可以在重组蛋白生产系统中通过表达病毒表面蛋白来生产，这些蛋白自发组装成类似于真实病毒或亚病毒颗粒的颗粒结构。 VLP 是开发安全有效的疫苗和诊断抗原的绝佳平台。在各种重组蛋白生产系统中，杆状病毒-昆虫细胞系统已广泛用于生产各种VLP。该系统已用于生产获得许可的人乳头瘤病毒样颗粒疫苗。然而，杆状病毒-昆虫细胞系统有一些固有的局限性，包括子代杆状病毒颗粒污染 VLP。稳定转化的昆虫细胞已成为杆状病毒-昆虫细胞系统的有吸引力的替代品。不同类型的 VLP（有或没有包膜、由单个或多个结构蛋白组成）已在稳定转化的昆虫细胞中产生。由稳定转化的昆虫细胞产生的VLP已成功引发体内免疫反应。在某些情况下，用重组昆虫细胞获得的VLP产量与用杆状病毒感染的昆虫细胞获得的产量相当或更高。重组昆虫细胞为 VLP 的开发和生产提供了一种有前途的方法。
Virus-like particles (VLPs) can be produced in recombinant protein production systems by expressing viral surface proteins that spontaneously assemble into particulate structures similar to authentic viral or subviral particles. VLPs serve as excellent platforms for the development of safe and effective vaccines and diagnostic antigens. Among various recombinant protein production systems, the baculovirus–insect cell system has been used extensively for the production of a wide variety of VLPs. This system is already employed for the manufacture of a licensed human papillomavirus-like particle vaccine. However, the baculovirus–insect cell system has several inherent limitations including contamination of VLPs with progeny baculovirus particles. Stably transformed insect cells have emerged as attractive alternatives to the baculovirus–insect cell system. Different types of VLPs, with or without an envelope and composed of either single or multiple structural proteins, have been produced in stably transformed insect cells. VLPs produced by stably transformed insect cells have successfully elicited immune responses in vivo. In some cases, the yield of VLPs attained with recombinant insect cells was comparable to, or higher than, that obtained by baculovirus-infected insect cells. Recombinant insect cells offer a promising approach to the development and production of VLPs.