Tetrabromobisphenol A induced reproductive endocrine-disrupting effects in mussel Mytilus galloprovincialis.
Tetrabromobisphenol A induced reproductive endocrine-disrupting effects in mussel Mytilus galloprovincialis.
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DOI：
10.1016/j.jhazmat.2021.126228
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发表时间：
2021-08
影响因子：
13.6
通讯作者：
Shuang Wang;Chenglong Ji;Fei Li;Junfei Zhan;Tao Sun;Jianhui Tang;Huifeng Wu
中科院分区：
文献类型：
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作者：
Shuang Wang;Chenglong Ji;Fei Li;Junfei Zhan;Tao Sun;Jianhui Tang;Huifeng Wu
四溴双酚A（TBBPA）污染对海洋生物具有潜在的内分泌干扰作用，对海洋生物构成了极大的风险。然而，TBBPA对海洋无脊椎动物的内分泌干扰效应却很少受到关注。本研究通过观察TBBPA处理贻贝30 d后配子发育、性别特异性基因表达和脊椎动物性激素水平的变化，研究TBBPA对贻贝生殖内分泌的影响。此外，进行转录组学分析和酶活性研究生殖内分泌干扰作用的潜在机制。我们发现，促进配子发生和脊椎动物性激素的改变发生在TBBPA处理的两性贻贝。同时，雌激素磺基转移酶（SULT1E1）和类固醇硫酸酯酶（STS）在转录水平上被TBBPA处理后上调，表明TBBPA通过促进类固醇的磺化和硫酸酯的水解而破坏了贻贝的类固醇合成。TBBPA生物转化的SULT的诱导可能是类固醇合成和类固醇代谢失调的原因。总之，这些发现为评估TBBPA的影响以及TBBPA在海洋环境中的生物监测提供了新的见解。
Tetrabromobisphenol A (TBBPA) pollution in marine environmental media poses great risks to marine organisms due to its potential endocrine-disrupting effects. However, limited attention of TBBPA’s endocrine-disrupting effects has been paid on marine invertebrates. In this work, the reproductive endocrine-disrupting effects of TBBPA were evaluated by observing the gametes development, quantifying the gender-specific gene expression, and determining vertebrate sex hormones in musselsMytilus galloprovincialistreated with TBBPA for 30 days. Additionally, transcriptomic profiling and enzymes activities were conducted to investigate the potential mechanisms of reproductive endocrine-disrupting effects. We found that promotion of gametogenesis and alterations of vertebrate sex hormones occurred in TBBPA-treated mussels of both sexes. Meanwhile, estrogen sulfotransferase (SULT1E1) and steroid sulfatase (STS) were up-regulated at transcript level as a result of TBBPA treatments, suggesting that TBBPA disrupted the steroidogenesis in mussels through promoting steroids sulfonation and hydrolysis of sulfate steroids. The induction of SULTs for TBBPA biotransformation might be responsible for the dysregulation of steroidogenesis and steroids metabolism. Overall, these findings provide a new insight into assessing impact of TBBPA as well as TBBPA biomonitoring in marine environment.