Kinetic Characterization by Surface Plasmon Resonance-Based Biosensors: Principle and Emerging Trends
Kinetic Characterization by Surface Plasmon Resonance-Based Biosensors: Principle and Emerging Trends
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DOI：
10.1007/s12195-008-0035-5
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发表时间：
2008-12-01
影响因子：
2.8
通讯作者：
Jolicoeur, Mario
中科院分区：
文献类型：
--
作者：
De Crescenzo, Gregory;Boucher, Cyril;Jolicoeur, Mario
在过去的三十年里，基于表面等离子体共振（SPR）的光学生物传感器已经成为研究生物分子相互作用的主要工具。该技术的迅速传播为研究人员提供了多功能和灵敏的仪器，用于识别与大分子相互作用有关的动力学和热力学参数。这篇综述文章介绍了基本原则，以及经典的实验方法和数据处理技术相关的商业可用的仪器用于表征蛋白质-蛋白质相互作用。重点将放在新的实验趋势，旨在改善任何SPR动力学研究的两个重要方面，即，生物传感器表面上的蛋白质束缚以及数据收集的优化。
Within the last three decades, surface plasmon resonance (SPR)-based optical biosensors have emerged as leading instruments for the study of biomolecular interactions. The rapid dissemination of the technology has provided researchers with versatile and sensitive instruments for the identification of kinetic and thermodynamic parameters related to macromolecular interactions. This review article presents the basic principles, as well as the classical experimental approaches and data treatment techniques related to the use of commercially available instruments for the characterization of protein-protein interactions. Emphasis will be put on new experimental trends aiming at improving two important aspects of any SPR kinetic studies, i.e., proteins tethering on biosensor surfaces as well as the optimization of data collection.