Interleukin-4 is not critical to pathogenesis in a mouse model of Pseudomonas aeruginosa corneal infection
Interleukin-4 is not critical to pathogenesis in a mouse model of Pseudomonas aeruginosa corneal infection
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DOI：
10.1080/02713680590968583
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发表时间：
2005-07-01
影响因子：
2
通讯作者：
Willcox, MDP
中科院分区：
文献类型：
--
作者：
Cole, N;Hume, E;Willcox, MDP
目的：目的探讨白细胞介素-4（IL-4）在绿脓杆菌角膜感染小鼠模型中对初始宿主反应的作用。方法：用铜绿假单胞菌局部攻击6至8周龄IL-4-/-和野生型小鼠的角膜。在攻击后24小时和7天收集眼组织。进行活菌计数、髓过氧化物酶测定、细胞因子水平以及临床和组织学检查。结果如下：在用铜绿假单胞菌激发期间，在任一时间点均未观察到IL-4-/-和野生型小鼠之间的临床、组织学或细菌载量差异。然而，观察到IL-6、KC和IL-10的细胞因子水平的差异。结论：所提供的数据表明，IL-4，一种中心Th 2细胞因子，在感染过程的早期阶段，可能对铜绿假单胞菌细菌性角膜炎模型的发病机制或细菌清除并不重要。
Purpose: To determine the contribution of interleukin-4 (IL-4) to the initial host response during corneal infection with Pseudomonas aeruginosa in a mouse model. Methods: Corneas of 6- to 8- week- old IL-4-/- and wild-type mice were topically challenged with P. aeruginosa. Ocular tissue was collected 24 hr and 7 days postchallenge. Viable bacterial counts, myeloperoxidase assays, cytokine levels, and clinical and histological examinations were performed. Results: During challenge with P. aeruginosa, no differences were observed clinically, histologically, or in bacterial load between IL-4-/- and wild-type mice at either time point. However, differences in cytokine levels of IL-6, KC, and IL-10 were observed. Conclusions: The data presented indicate that IL-4, a central Th2 cytokine, may not be critical to the pathogenesis or bacterial clearance in this model of P. aeruginosa bacterial keratitis during the early stages of the infectious process.