A comparative analysis of antibacterial properties and inflammatory responses for the KR-12 peptide on titanium and PEGylated titanium surfaces
A comparative analysis of antibacterial properties and inflammatory responses for the KR-12 peptide on titanium and PEGylated titanium surfaces
复制标题
钛和聚乙二醇化钛表面 KR-12 肽的抗菌特性和炎症反应的比较分析
DOI：
10.1039/c7ra05538b
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Yue, Bing
中科院分区：
文献类型：
--
作者：
Nie, Bin'en;Long, Teng;Yue, Bing
抗微生物肽在钛（Ti）表面上的共价固定已被广泛地进行以抑制细菌粘附和生物膜形成，然而，尚不清楚在该过程中间隔物是否是抗细菌粘附所必需的，同时仍表现出良好的生物相容性。我们研究了钛和聚乙二醇化钛与共价固定KR-12肽表面的抗菌性能和炎症反应。KR-12肽衍生自LL-37，其在溶液中具有杀菌和抑菌作用。通过X射线光电子能谱法、接触角测量和原子力显微镜测定成功的共价固定。KR-12掺入显著降低了细菌对钛表面的粘附。与Ti-KR-12相比，通过PEG间隔物固定KR-12提高了抗菌效率，并减少了表皮葡萄球菌的体外粘附和生物膜形成。在钛表面上观察到从具有长丝状伪足的THP-1细胞去除的活化的巨噬细胞，这与TNF-α和IL-1β分泌增加一致。KR-12修饰的Ti表面和PEG化的Ti表面显著降低TNF-α和IL-1β的分泌，并且巨噬细胞保持在灭活的圆形状态。在KR-12固定在Ti表面上之前，PEG间隔物的接枝改善了抗菌性能并降低了巨噬细胞活化，并且还可以降低总体炎症反应。因此，这种方法显示出生物功能化Ti以抗细菌粘附和减少巨噬细胞炎症的潜力。
Covalent immobilisation of antimicrobial peptides on titanium (Ti) surfaces has been widely performed to inhibit bacterial adhesion and biofilm formation, however, it is unclear whether a spacer is necessary in this process for anti-bacteria adhesion, while still exhibiting good biocompatibility. We investigated the antibacterial properties and inflammatory response of Ti and PEGylated Ti with covalently immobilized KR-12 peptide on the surfaces. The KR-12 peptide was derived from LL-37, which is bactericidal and bacteriostatic in solution. Successful covalent immobilisation was determined by X-ray photoelectron spectrometry, contact angle measurements, and atomic force microscopy. KR-12 incorporation significantly decreased the bacterial adhesion to the Ti surface. Compared to the Ti-KR-12, the KR-12 immobilisation via a PEG spacer increased anti-microbial efficiency and reduced in vitro adhesion and biofilm formation of Staphylococcus epidermidis. Activated macrophages deprived from THP-1 cells with long filopodia were observed on the titanium surface, which was in agreement with increased TNF-α and IL-1β secretion. The KR-12-modified Ti surface and PEGylated Ti surface significantly decreased TNF-α and IL-1β secretion and macrophages remained in inactivated round states. The grafting of a PEG spacer prior to KR-12 immobilisation on the Ti surface improved the antibacterial properties and reduced macrophage activation, and may also decrease overall inflammatory responses. Thus, this approach showed the potential to biofunctionalize Ti for anti-bacteria adhesion and reducing macrophage inflammation.