Photo-control of excitation waves in cardiomyocyte tissue culture.
Photo-control of excitation waves in cardiomyocyte tissue culture.
复制标题
心肌细胞组织培养中激发波的光控制。
作者：
N. Magome;G. Kanaporis;N. Moisan;Koichiro Tanaka;K. Agladze
偶氮苯光开关最近被报道用于控制水蚤的神经细胞活动和心跳。在这里，我们报告了通过添加偶氮苯三甲基溴化铵(AzoTAB)来光控制已被光敏的培养心肌细胞的兴奋。AzoTAB的反式异构体可逆地抑制自发活动和兴奋波的传播，而顺式异构体对心肌细胞的电特性没有明显的影响。将光照波长λ从440 nm(反式异构体)切换到~350 nm(顺式异构体)，实现了偶氮染料的光异构化。在适当选择强度的两个波长的同时照射允许动态地控制顺反异构体的比率以及从正常到完全不可兴奋的兴奋性水平。用AzoTAB处理的新生大鼠心肌细胞融合单层进行实验。用钙离子敏感的荧光染料Fluo-4监测激发波。通过将双波长照明图案投射到其他均匀的细胞层上，我们能够创建具有所需拓扑、尺寸和功能属性的可激发网络。本文讨论了这项技术在分析复杂的电兴奋和心律失常模式方面的潜在应用。
Azobenzene photoswitches were recently reported to control the activity of neural cells and heart beat in leeches. Here, we report photocontrol of excitation of cultured cardiomyocytes that have been made light sensitive by using the addition of azobenzene trimethylammonium bromide (AzoTAB). The trans-isomer of AzoTAB reversibly suppresses spontaneous activity and propagation of excitation waves, whereas the cis-isomer has no detectable effect on the electrical properties of cardiomyocytes. Photoisomerization of AzoTAB was achieved by switching the illumination wavelength, λ, from ~440 nm (trans-isomer) to ~350 nm (cis-isomer). Simultaneous irradiation at two wavelengths with properly chosen intensities allowed for dynamic control of the cis-isomer/trans-isomer ratio and the level of excitability from normal to fully unexcitable. Experiments were conducted by using AzoTAB-treated confluent monolayers of neonatal rat cardiomyocytes. Excitation waves were monitored by using the Ca2+-sensitive fluorescent dye Fluo-4. By projecting two-wavelength illumination patterns onto otherwise uniform cell layers, we were able to create excitable networks with the desired topology, dimensions, and functional properties. The present article discusses potential applications of this technique for the analysis of complex patterns of electrical excitation and cardiac arrhythmias.
DOI：
10.1152/physiol.00018.2008
发表时间：
2008-10
期刊：
Physiology (Bethesda, Md.)
影响因子：
--
作者：
Gorostiza P;Isacoff EY
通讯作者：
Isacoff EY
培养皿中的纤颤/除颤成像。
影响因子：
1.3
作者：
Tung,Leslie;Cysyk,Joshua
通讯作者：
Cysyk,Joshua
影响因子：
16.6
作者：
Banghart, Matthew R.;Mourot, Alexandre;Fortin, Doris L.;Yao, Jennifer Z.;Kramer, Richard H.;Trauner, Dirk
通讯作者：
Trauner, Dirk