Meal-induced 24-hour profile of circulating glycated insulin in type 2 diabetic subjects measured by a novel radioimmunoassay
Meal-induced 24-hour profile of circulating glycated insulin in type 2 diabetic subjects measured by a novel radioimmunoassay
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DOI：
10.1053/meta.2003.50150
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发表时间：
2003-05-01
影响因子：
9.8
通讯作者：
O'Harte, FPM
中科院分区：
文献类型：
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作者：
Lindsay, JR;McKillop, AM;O'Harte, FPM
越来越多的证据支持糖化胰岛素在 2 型糖尿病的胰岛素抵抗状态中发挥作用。我们使用新型放射免疫分析 (RIA) 测量了血浆糖化胰岛素的 24 小时概况，以评估进餐刺激和间歇性禁食对 2 型糖尿病患者血浆糖化胰岛素循环浓度的影响。患者（n = 6；血红蛋白 A(1c) [HbA(1c)]，7.2% +/- 0.6%；空腹血糖，7.4 +/- 0.7 mmol/L；体重指数 [BMI]，35.7 +/- 3.5 kg/m(2)；年龄，56.3 +/- 4.4 岁）入院 24 小时并接受标准化膳食方案。上午 8 点至午夜以及每晚 2 小时采集血浆糖化胰岛素、葡萄糖、胰岛素和 C 肽浓度，每半小时采集一次静脉样本。 24 小时内平均血浆糖化胰岛素浓度为 27.8 +/- 1.2 pmol/L，胰岛素：糖化胰岛素的平均比例为 11:1。循环葡萄糖、胰岛素、C肽和糖化胰岛素遵循基础和膳食相关模式，分别在早餐、午餐和晚餐后增加最显着。早上、下午、晚上和夜间时段的糖化胰岛素平均浓度分别为 24.4 +/- 2.5、28.7 +/- 2.3、31.1 +/- 2.1 和 26.2 +/- 1.5 pmol/L，与对照组相比，胰岛素与糖化胰岛素的摩尔比明显更高，分别为 18.0:1、14.2:1 和 12.7:1。夜间 7.01（P < .01 至 P < .001）。这些数据表明，糖化胰岛素在 2 型糖尿病中以相对较高的浓度循环，具有基础和膳食刺激释放的昼夜模式。夜间循环的糖化胰岛素比例较高，表明与天然胰岛素相比代谢清除率存在差异。 (C) 2003 Elsevier Inc. 保留所有权利。
Increasing evidence supports a role for glycated insulin in the insulin-resistant state of type 2 diabetes. We measured 24-hour profiles of plasma glycated insulin, using a novel radioimmunoassay (RIA), to evaluate the effects of meal stimulation and intermittent fasting on circulating concentrations of plasma glycated insulin in type 2 diabetes. Patients (n = 6; hemoglobin A(1c) [HbA(1c)], 7.2% +/- 0.6%; fasting plasma glucose, 7.4 +/- 0.7 mmol/L; body mass index [BMI], 35.7 +/- 3.5 kg/m(2); age, 56.3 +/- 4.4 years) were admitted for 24 hours and received a standardized meal regimen. Half-hourly venous samples were taken for plasma glycated insulin, glucose, insulin, and C-peptide concentrations between 8 Am and midnight and 2-hourly overnight. The mean plasma glycated insulin concentration over 24 hours was 27.8 +/- 1.2 pmol/L with a mean ratio of insulin:glycated insulin of 11:1. Circulating glucose, insulin, C-peptide, and glycated insulin followed a basal and meal-related pattern with most prominent increments following breakfast, lunch, and evening meal, respectively. The mean concentrations of glycated insulin during the morning, afternoon, evening, and night-time periods were 24.4 +/- 2.5, 28.7 +/- 2.3, 31.1 +/- 2.1, and 26.2 +/- 1.5 pmol/L, respectively, giving significantly higher molar ratios of insulin:glycated insulin of 18.0:1, 14.2:1, and 12.7:1 compared with 7.01 at night (P < .01 to P < .001). These data demonstrate that glycated insulin circulates at relatively high concentrations in type 2 diabetes with a diurnal pattern of basal and meal-stimulated release. A higher proportion of glycated insulin circulates at night suggestive of differences in metabolic clearance compared with native insulin. (C) 2003 Elsevier Inc. All rights reserved.