Response of cells to hyperthermia under acute and chronic hypoxic conditions.
Response of cells to hyperthermia under acute and chronic hypoxic conditions.
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DOI：
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发表时间：
1979-03
期刊：
影响因子：
11.2
通讯作者：
L. Gerweck;T. Nygaard;M. Burlett
中科院分区：
文献类型：
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作者：
L. Gerweck;T. Nygaard;M. Burlett
在缺氧条件下培养0至30小时后，测定加热至42 ℃的中国仓鼠细胞的致死反应。氧合和急性缺氧的细胞同样敏感的高温，然而，敏感性增加的时间在缺氧条件下培养治疗前。在42度下3小时导致在急性缺氧条件下存活分数约等于0.1，并且对于在热处理之前在缺氧条件下培养30小时的细胞，存活分数小于0.001。对高温的敏感性增加部分是由于细胞代谢导致的培养基pH值降低;这可以通过在热处理前立即将pH值增加至7.3来逆转。然而，即使在完全受控的pH条件下，长时间的缺氧也会使高温细胞杀伤增加约5倍或等于5倍。在治疗前将细胞恢复到正常氧分压并不能逆转这种效应。这些数据表明，肿瘤样微环境条件（降低O2张力和pH值）大大增加了细胞对42度高温的敏感性。
The lethal response of Chinese hamster cells heated to 42 degrees was determined following 0 to 30 hr culturing under hypoxic conditions. Oxygenated and acutely hypoxic cells were equally sensitive to hyperthermia; however, sensitivity increased with the time of culturing under hypoxic conditions prior to treatment. Three hr at 42 degrees resulted in a surviving fraction of approximately or equal to 0.1 under acute hypoxic conditions and less than 0.001 for cells cultured for 30 hr under oxygen-deprived conditions before the heat treatment. The increased sensitivity to hyperthermia was was due in part to a decrease in the pH of the medium which occurred as a result of cell metabolism; this could be reversed by increasing pH to 7.3 immediately prior to heat treatment. However, even under fully controlled pH conditions, prolonged oxygen deprivation increased hyperthermic cell killing by a factor of approximately or equal to 5. This effect was not reversed by returning the cells to normal oxygen tension prior to treatment. These data demonstrate that tumorlike microenvironmental conditions (reduced O2 tension and pH) substantially increase the sensitivity of cells to 42 degrees hyperthermia.