Aeromonas fluvialis sp nov., isolated from a Spanish river
Aeromonas fluvialis sp nov., isolated from a Spanish river
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DOI：
10.1099/ijs.0.011643-0
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Figueras, Maria J.
中科院分区：
文献类型：
--
作者：
Alperi, Anabel;Martinez-Murcia, Antonio J.;Figueras, Maria J.
从采集自西班牙东北部赫罗纳Muga河的水样中分离出一种革兰氏染色阴性的兼性厌氧细菌菌株，命名为717（T）。16 S rRNA基因序列的初步分析表明，该菌株属于气单胞菌属，最近的物种是Veronii气单胞菌（99.5%的相似性，有7个不同的核苷酸）。基于5个管家基因（gyrB、rpoD、recA、dnaJ和gyrA; 36134 bp）的多位点系统发育分析的多相研究表明，分离物717（T）是一个独立的系统发育线，与温和气单胞菌、Veronii气单胞菌和嗜别糖气单胞菌是最近的邻居种。DNA-DNA重组实验和表型分析表明，菌株717（T）代表一个新种，建议将其命名为河流气单胞菌（Aeromonasfluvialis sp. nov.），模式菌株717（T）（=CECT 7401（T）=LMG 24681（T））。
A Gram-stain-negative, facultatively anaerobic bacterial strain, designated 717(T), was isolated from a water sample collected from the Muga river, Girona, north-east Spain. Preliminary analysis of the 16S rRNA gene sequence showed that this strain belonged to the genus Aeromonas, the nearest species being Aeromonas veronii (99.5% similarity, with seven different nucleotides). A polyphasic study based on a multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 36134 bp) showed isolate 717(T) to be an independent phylogenetic line, with Aeromonas sobria, Aeromonas veronii and Aeromonas allosaccharophila as the closest neighbour species. DNA-DNA reassociation experiments and phenotypic analysis identified that strain 717(T) represents a novel species, for which the name Aeromonas fluvialis sp. nov. is proposed, with type strain 717(T) (=CECT 7401(T) =LMG 24681(T)).