Distinct intramembrane cleavage of the β-amyloid precursor protein family resembling γ-secretase-like cleavage of Notch
Distinct intramembrane cleavage of the β-amyloid precursor protein family resembling γ-secretase-like cleavage of Notch
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DOI：
10.1074/jbc.c100357200
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发表时间：
2001-09-21
影响因子：
4.8
通讯作者：
Ihara, Y
中科院分区：
文献类型：
--
作者：
Gu, YJ;Misonou, H;Ihara, Y
β-淀粉样前体蛋白的膜内裂解是β-淀粉样蛋白生成的最后一步。随之而来的是59或57个残基的C末端片段，称为CTF伽马。对大鼠脑膜制剂中可能产生的CTFγ进行了纯化和测序。用较短的50个和49个残基片段代替了CTF伽马。此外，我们还发现了类似的β-淀粉样前体蛋白1和2的C末端片段；这些片段也在相应的位置被切割。这一新发现的切割发生在细胞膜边界内的2-5个残基位置，与Notch 1的伽马分泌酶样切割非常相似。
The intramembrane cleavage of beta -amyloid precursor protein by gamma -secretase is the final step in the generation of amyloid beta -protein. A 59- or 57-residue C-terminal fragment called CTF gamma is produced concomitantly. Putative CTF gamma generated in rat brain membrane preparations was purified and sequenced. Instead of CTF gamma, shorter 50- and 49-residue fragments were identified. In addition, we found similar C-terminal fragments of beta -amyloid precursor-like proteins 1 and 2; these were also cleaved at corresponding sites. This newly identified cleavage occurs at a site two to five residues inside the cytoplasmic membrane boundary, which is very similar to gamma -secretase-like cleavage of Notch 1.