Aggregation-induced emission nanoparticles as photosensitizer for two-photon photodynamic therapy
Aggregation-induced emission nanoparticles as photosensitizer for two-photon photodynamic therapy
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DOI：
10.1039/c7qm00092h
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发表时间：
2017-09-01
影响因子：
7
通讯作者：
Qian, Jun
中科院分区：
文献类型：
--
作者：
Alifu, Nuernisha;Dong, Xiaobiao;Qian, Jun
双光子激发荧光显微术和纳米粒子辅助光动力学疗法（PDT）是生物医学研究的两个重要领域，它们的结合可以带来更多的益处。合成了一种具有聚集诱导发光（AIE）特性的红色光敏剂四苯乙烯（TPE-red），并将其与聚苯乙烯-马来酸酐共聚物（PSMA）包覆在一起形成纳米粒子。双光子荧光，以及双光子激发活性氧（ROS）的产生由TPE红色PSMA纳米粒子，其特征在于。利用飞秒激光在1040 nm处观察到了较大的双光子吸收截面。在1040 nm fs激光激发下，使用TPE-红色-PSMA纳米颗粒作为PS，在肿瘤细胞上很好地实现了经由双光子激发的PDT。基于我们的研究，我们认为，具有AIE活性PS的双光子激发光动力学疗法在深部组织成像引导治疗中具有巨大的应用潜力。
Two-photon excited fluorescence microscopy and nanoparticle-assisted photodynamic therapy (PDT) are two important areas in biomedical research, and their combination can be more beneficial. A type of red emissive photosensitizer (PS) with aggregation-induced emission (AIE) features, which is called tetraphenylethylene (TPE-red), was synthesized and further encapsulated with poly(styrene-co-maleic anhydride) (PSMA) to form nanoparticles. Two-photon fluorescence, as well as two-photon excited reactive oxygen species (ROS) generation by TPE-red-PSMA nanoparticles, was characterized. A large two-photon absorption cross-section was observed at 1040 nm with a femtosecond (fs) laser. PDT via two-photon excitation was well realized on tumor cells, using TPE-red-PSMA nanoparticles as PSs under 1040 nm fs laser excitation. Based on our study, we believe that two-photon excited PDT with AIE-active PSs has great potential applications in deep tissue imaging-guided therapy.