Enhancement of cytotoxicity of modeccin by nigericin in modeccin-resistant mutant cell lines.
Enhancement of cytotoxicity of modeccin by nigericin in modeccin-resistant mutant cell lines.
复制标题
尼日利亚菌素在Modeccin抗性突变细胞系中增强Modeccin的细胞毒性。
DOI：
10.1016/0014-4827(88)90310-2
复制
发表时间：
1988
影响因子：
3.7
通讯作者：
Wu,HC
中科院分区：
文献类型：
--
作者：
Ghosh,PC;Wu,HC
We have isolated a Chinese hamster ovary cell mutant (DMPR-2) simultaneously resistant to diphtheria toxin and modeccin. In addition to the increased resistance to these two toxins used in the selection, this mutant is more resistant toPseudomonastoxin and hypersensitive to ricin than the parental cell line. In contrast to the wild-type cells in which nigericin protects cells from modeccin, the cytotoxicity of modeccin in the DMPR-2 mutant is enhanced by nigericin. We have also studied the effects of nigericin and NH4Cl on the cytotoxicity of modeccin in a modeccin-resistant mutant of HeLa cells (ModRI). The cytotoxicity of modeccin is enhanced by nigericin in ModRI mutant cells, in contrast to the protection of modeccin cytotoxicity by nigericin in the parental HeLa cells. Our results suggest that modeccin can reach the cytosol of mammalian cells by two distinct routes; the major route requires endosomal acidification and the minor route is activated by nigericin.
