Long non-coding RNA PVT1-5 promotes cell proliferation by regulating miR-126/SLC7A5 axis in lung cancer
Long non-coding RNA PVT1-5 promotes cell proliferation by regulating miR-126/SLC7A5 axis in lung cancer
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DOI：
10.1016/j.bbrc.2017.12.114
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Shangyong Zheng
中科院分区：
文献类型：
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作者：
Hongliang Li;Shuxian Chen;Jia Liu;Xiaopeng Guo;Xudong Xiang;Tianqi Dong;Pengzhan Ran;Qian Li;Bei Zhu;Xiyu Zhang;Dan Wang;Chunjie Xiao;Shangyong Zheng
Dysregulated long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play key roles in the devel-.opment of human cancers. The lncRNA plasmacytoma variant translocation 1 (PVT1) is reported to be an.oncogene in a variety of cancers. However, the roles of PVT1-5 and its related miRNAs in lung cancer are.poorly understood. In this study, we found that PVT1-5 expression was significantly increased in lung.cancer tissues and cell lines. By using biotin-labeled lncRNA-PVT1-5 probe for miRNA in vivo precipi-.tation (miRIP) in lung cancer cells and dual-luciferase reporterassays, we identified that miR-126 was.associated with lncRNA-PVT1-5. Furthermore, knockdown of lncRNA-PVT1-5 in cells could down-.regulate the expression of SLC7A5, the target of oncogenic miR-126, resulting in the cell proliferation..Conversely, inhibiting the expression of miR-126 markedly increased the expression of SLC7A5 and.alleviated cell proliferation inhibition. Thus, our results indicated that lncRNA-PVT1-5 may function as a.competing endogenous RNA (ceRNA) for miR-126 to promote cell proliferation by regulating the miR-.126/SLC7A5 pathway, suggesting that lncRNA-PVT1-5 plays a crucial role in lung cancer progression.and lncRNA-PVT1-5/miR-126/SLC7A5 regulatory network may shed light on tumorigenesis in lung.cancer.
