A sandwich-type DNA biosensor based on electrochemical co-reduction synthesis of graphene-three dimensional nanostructure gold nanocomposite films
A sandwich-type DNA biosensor based on electrochemical co-reduction synthesis of graphene-three dimensional nanostructure gold nanocomposite films
复制标题
基于石墨烯-三维纳米结构金纳米复合薄膜电化学共还原合成的夹心型DNA生物传感器
DOI：
10.1016/j.aca.2012.12.049
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发表时间：
2013-03-12
影响因子：
6.2
通讯作者：
Yang, Shu-Yu
中科院分区：
文献类型：
--
作者：
Liu, Ai-Lin;Zhong, Guang-Xian;Yang, Shu-Yu
制备了一种基于石墨烯-三维纳米结构金纳米复合材料修饰玻碳电极（G-3D Au/GCE）的新型电化学DNA生物传感器，用于检测与骨肉瘤相关的生存素基因。 G-3D Au 薄膜是通过氧化石墨和 HAuCl4 在阴极电位下一步电化学共还原制备的。 G-3D Au/GCE的活性表面积为2.629 cm(2)，是相同实验条件下Au涂层GCE的约3.8倍，是具有相似几何面积的平面金电极的8.8倍。通过扫描电子显微镜（SEM）、循环伏安法（CV）和电化学阻抗谱（EIS）对所得纳米复合材料进行了表征，该复合材料具有高导电性、电催化性和生物相容性。该电化学DNA生物传感器采用“三明治型”检测策略，通过CV和电流-时间曲线检测来测量该DNA生物传感器的响应。在最佳条件下，电流信号与互补DNA浓度的对数函数在50-5000 fM范围内呈良好的线性关系，检测限为3.4 fM。这种新型生物传感器表现出快速的电流响应、高灵敏度和选择性，并已用于现实生活样品的聚合酶链反应检测，取得了令人满意的结果。 (C) 2013 Elsevier B.V. 保留所有权利。
A novel electrochemical DNA biosensor based on graphene-three dimensional nanostructure gold nanocomposite modified glassy carbon electrode (G-3D Au/GCE) was fabricated for detection of survivin gene which was correlated with osteosarcoma. The G-3D Au film was prepared with one-step electrochemical coreduction with graphite oxide and HAuCl4 at cathodic potentials. The active surface area of G-3D Au/GCE was 2.629 cm(2), which was about 3.8 times compared to that of a Au-coated GCE under the same experimental conditions, and 8.8 times compared to a planar gold electrode with a similar geometric area. The resultant nanocomposites with high conductivity, electrocatalysis and biocompatibility were characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A "sandwich-type" detection strategy was employed in this electrochemical DNA biosensor and the response of this DNA biosensor was measured by CV and amperometric current-time curve detection. Under optimum conditions, there was a good linear relationship between the current signal and the logarithmic function of complementary DNA concentration in a range of 50-5000 fM with a detection limit of 3.4 fM. This new biosensor exhibited a fast amperometric response, high sensitivity and selectivity and has been used in a polymerase chain reaction assay of real-life sample with a satisfactory result. (C) 2013 Elsevier B.V. All rights reserved.