Glutathione and catalase provide overlapping defenses for protection against hydrogen peroxide in the yeast Saccharomyces cerevisiae
Glutathione and catalase provide overlapping defenses for protection against hydrogen peroxide in the yeast Saccharomyces cerevisiae
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DOI：
10.1006/bbrc.1998.9864
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发表时间：
1998-12-30
影响因子：
3.1
通讯作者：
Dawes, IW
中科院分区：
文献类型：
--
作者：
Grant, CM;Perrone, G;Dawes, IW
谷胱甘肽 (GSH) 是一种丰富且普遍存在的低分子量硫醇，它在许多细胞过程中发挥作用，包括防止活性氧的有害影响。我们的实验探讨了谷胱甘肽在酿酒酵母中对抗过氧化氢的作用，并表明谷胱甘肽和过氧化氢酶提供了重叠的防御系统。 GSH 似乎是防止过氧化氢的主要抗氧化剂，因为缺乏 GSH (gsh1) 或谷胱甘肽还原酶 (glr1) 的突变体是敏感的，而缺乏过氧化氢酶 A (cta1) 或过氧化氢酶 T (ctt1) 的菌株对此氧化剂的抵抗力不受影响。此外，用过氧化氢处理后，氧化的、蛋白质结合的和细胞外的谷胱甘肽的水平都增加，但细胞内的谷胱甘肽减少了。然而，有两方面的证据表明在没有谷胱甘肽的情况下需要过氧化氢酶；首先，同时缺乏过氧化氢酶 A 和 T 的菌株在用过氧化氢处理后积累的氧化型谷胱甘肽水平增加；其次，过氧化氢酶基因的缺失加剧了 glr1 和 gsh1 突变体的过氧化氢敏感性。 (C) 1998 年学术出版社。
Glutathione (GSH) is an abundant and ubiquitous low-molecular-weight thiol which has proposed roles in many cellular processes including protection against the deleterious effects of reactive oxygen species. Our experiments have addressed the role of GSH in protection against hydrogen peroxide in the yeast Saccharomyces cerevisiae, and have shown that GSH and catalase provide overlapping defense systems. GSH appears to be the primary antioxidant for protection against hydrogen peroxide since mutants lacking GSH (gsh1) or glutathione reductase (glr1) are sensitive, whereas, strains lacking catalase A (cta1) or catalase T (ctt1) are unaffected in resistance to this oxidant. Furthermore, following treatment with hydrogen peroxide, the levels of oxidized, protein-bound and extracellular GSH were all increased at the expense of intracellular GSH. However, there are two lines of evidence that indicate catalases are required in the absence of GSH; firstly, strains that lack both catalase A and T accumulate increased levels of oxidized glutathione following treatment with hydrogen peroxide; and secondly, deletion of catalase genes exacerbates the hydrogen peroxide sensitivity of glr1 and gsh1 mutants. (C) 1998 Academic Press.