Routine Digital Pathology Workflow: The Catania Experience.
Routine Digital Pathology Workflow: The Catania Experience.
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DOI：
10.4103/jpi.jpi_58_17
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发表时间：
2017
影响因子：
--
通讯作者：
Rossi ED
中科院分区：
文献类型：
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作者：
Fraggetta F;Garozzo S;Zannoni GF;Pantanowitz L;Rossi ED
全幻灯片成像(WSI)在常规临床实践中的成功实施仅在世界范围内的少数几个病理学实验室完成。我们报告了在意大利卡塔尼亚坎尼扎罗医院的病理实验室向有效和完整的数字外科病理学工作流程的过渡。所有(100%)永久性组织病理学玻片均在Aperio AT2扫描仪上以×20的速度数字化。采用兼容染色和扫描载玻架，简化了操作。ESlide Manager软件与解剖病理学实验室信息系统双向接口。连接到二维(2D)条形码跟踪系统的虚拟载玻片托盘允许病理学家确认他们被正确分配了载玻片，并且这些玻璃片上的所有组织都被扫描了。超过115,000张玻璃片被数字化，扫描失败率约为1%。在扫描前烘干玻璃片可以最大限度地减少粘在扫描仪机架上的玻璃片。实施需要引入二维条形码跟踪系统和修改组织学工作流程。我们的经验表明，有效地将WSI用于主要诊断用途更依赖于优化成像前变量和与实验室信息系统的集成，而不是信息技术基础设施和确保病理学家的认可。将数字病理学用于常规实践不仅利用了数字成像的好处，而且为在病理实验室建立工作流程标准化创造了机会。
Successful implementation of whole slide imaging (WSI) for routine clinical practice has been accomplished in only a few pathology laboratories worldwide. We report the transition to an effective and complete digital surgical pathology workflow in the pathology laboratory at Cannizzaro Hospital in Catania, Italy. All (100%) permanent histopathology glass slides were digitized at ×20 using Aperio AT2 scanners. Compatible stain and scanning slide racks were employed to streamline operations. eSlide Manager software was bidirectionally interfaced with the anatomic pathology laboratory information system. Virtual slide trays connected to the two-dimensional (2D) barcode tracking system allowed pathologists to confirm that they were correctly assigned slides and that all tissues on these glass slides were scanned. Over 115,000 glass slides were digitized with a scan fail rate of around 1%. Drying glass slides before scanning minimized them sticking to scanner racks. Implementation required introduction of a 2D barcode tracking system and modification of histology workflow processes. Our experience indicates that effective adoption of WSI for primary diagnostic use was more dependent on optimizing preimaging variables and integration with the laboratory information system than on information technology infrastructure and ensuring pathologist buy-in. Implementation of digital pathology for routine practice not only leveraged the benefits of digital imaging but also creates an opportunity for establishing standardization of workflow processes in the pathology laboratory.