Synthesis of photoaffinity labeled activity-based protein profiling probe and production of natural TetR protein for immunoassay of tetracyclines in milk
Synthesis of photoaffinity labeled activity-based protein profiling probe and production of natural TetR protein for immunoassay of tetracyclines in milk
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光亲和标记活性蛋白分析探针的合成和天然 TetR 蛋白的生产用于牛奶中四环素的免疫分析
DOI：
10.1016/j.microc.2021.106779
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发表时间：
2021-11
影响因子：
4.8
通讯作者：
Liu Jing
中科院分区：
文献类型：
--
作者：
Xia Wan Qiu;Cui Peng Lei;Wang Jian Ping;Liu Jing
在本研究中，首次合成了一种基于光亲和标记活性的四环素药物蛋白谱探针，用于从大肠杆菌中捕获一种天然的四环素蛋白。TetR同时识别出10种四环素类药物。然后将其作为识别试剂，建立了一种直接竞争化学发光免疫分析法，用于牛奶中10种药物的检测。这些药物的ic50值范围为0.084 ~ 0.32 ng/mL，检出限范围为2.0 ~ 9.0 pg/mL。加样回收率为68.4% ~ 91.3%。经比较，天然TetR蛋白和所建立的方法比以往的TetR蛋白和相关的免疫测定具有更好的性能。因此，该方法可作为常规筛选牛奶中四环素类药物残留的实用工具。此外，这种类型的探针也可以用来生产受体和开发不同的免疫分析其他类别的药物。
In this study, a photoaffinity labeled activity-based protein profiling probe for tetracycline drugs was first synthesized that was used to capture a type of natural TetR protein fromEscherichia coli. The TetR simultaneously recognized 10 tetracycline drugs. Then it was used as recognition reagent to develop a direct competitive chemiluminescence immunoassay for detection of the 10 drugs in milk. The IC50values for these drugs were in the range of 0.084–0.32 ng/mL, and the limits of detection were in the range of 2.0–9.0 pg/mL. Their recoveries from the standards fortified blank milk samples were in the range of 68.4%-91.3%. After comparison, the natural TetR protein and the developed method showed better performances than the previous TetR proteins and the related immunoassays. Therefore, this method could be used as a practical tool for routine screening the residues of tetracycline drugs in milk. Furthermore, this type of probe could also be used to produce the receptors and develop different immunoassays for other classes of drugs.
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影响因子：
3.9
作者：
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通讯作者：
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影响因子：
1.8
作者：
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通讯作者：
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2.1
作者：
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通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
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