Cellular, biochemical and molecular characterization of the bovine temporomandibular joint disc
Cellular, biochemical and molecular characterization of the bovine temporomandibular joint disc
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DOI：
10.1016/s0003-9969(96)00068-4
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发表时间：
1996-08-01
影响因子：
3
通讯作者：
Puzas, JE
中科院分区：
文献类型：
--
作者：
Landesberg, R;Takeuchi, E;Puzas, JE
对牛颞下颌关节盘的细胞和胶原成分进行了分离和分析。在椎间盘的中央区域，发现了大量的I、II、IX和XII型胶原，这些分子的身份用胶原酶消化、Western印迹分析和北方印迹分析（对于II型胶原）进行了验证。从颞下颌关节盘分离的细胞在培养中合成碱性磷酸酶，蛋白多糖和胶原蛋白，然而，这些分子的基础合成率低于分离的成骨细胞，关节和生长板软骨细胞。TMJ椎间盘细胞在培养中比成骨细胞或软骨细胞增殖更快。转化生长因子β刺激增殖250%，而前列腺素E（2）没有影响。版权所有（C）1996 Elsevier Science Ltd.
The cellular and collagenous components of the bovine temporomandibular joint (TMJ) disc have been isolated and analysed. In the central regions of the disc, significant amounts of type I, II, IX and XII collagen were found. The identity of these molecules was verified with collagenase digestions, Western blot analysis and Northern blot analysis (for type II collagen). Cells isolated from the TMJ disc synthesized alkaline phosphatase, proteoglycans and collagen in culture; however, the basal rate of synthesis for these molecules was lower than that for isolated osteoblasts, articular and growth-plate chondrocytes. The TMJ disc cells proliferated more rapidly in culture than osteoblasts or chondrocytes. Transforming growth factor-beta stimulated proliferation by 250%, whereas prostaglandin E(2) had no effect. Copyright (C) 1996 Published by Elsevier Science Ltd.