Synergetic inhibition of daidzein and regular exercise on breast cancer in bearing-4T1 mice by regulating NK cells and apoptosis pathway.
Synergetic inhibition of daidzein and regular exercise on breast cancer in bearing-4T1 mice by regulating NK cells and apoptosis pathway.
复制标题
DOI：
10.1016/j.lfs.2020.117387
复制
发表时间：
2020-01
期刊：
影响因子：
6.1
通讯作者：
Bin Wang;Heshan Xu;Xiaoying Hu;Wenyu Ma;Jian Zhang;Yuanfeng Li;Min Yu;Yaru Zhang;Xuegang Li;Xiaoli Ye
中科院分区：
文献类型：
--
作者：
Bin Wang;Heshan Xu;Xiaoying Hu;Wenyu Ma;Jian Zhang;Yuanfeng Li;Min Yu;Yaru Zhang;Xuegang Li;Xiaoli Ye
本研究旨在探讨大豆黄酮和/或规律运动对乳腺癌的抑制作用及其可能的生物学机制。将小鼠乳腺癌细胞（4 T1）原位移植到BALB/c小鼠体内，经20 d（15 m/min，60 min/d）的常规运动训练后，再灌胃给予黄豆苷元（145 mg/kg），连续22 d。结果表明，运动或大豆黄酮对小鼠肿瘤生长有不同程度的抑制作用。运动与大豆苷元合用对肿瘤生长的抑制作用与对照组相比有显著的协同作用（P< 0.01）。进一步的研究表明，运动和大豆黄酮的联合作用通过上调肾上腺素和白细胞介素-6的水平，协同动员和重新分配自然杀伤细胞。此外，运动联合大豆黄酮通过Fas/FasL启动的线粒体凋亡信号通路诱导癌细胞凋亡。这些结果提示，规律运动结合大豆黄酮可能是预防和治疗乳腺癌的一种候选方法。
The aim of this study was to investigate the inhibition of daidzein or/and regular exercise on breast cancer and to reveal the potential biological mechanisms. BALB/c mice pretreated with regular exercise training for 20 days (15 m/min, 60 min/d) were orthotopically transplanted with mouse breast cancer cells (4T1), and then treated with daidzein (145 mg/kg) by gavage for another 22 days. Results showed that exercise or daidzein inhibited tumor growth in mice to a different degree. Particularly, co-treatment with exercise and daidzein showed an obviously synergistic inhibition on the tumor growth (P< 0.01), compared with the tumor control. Further researches indicated that the combination of exercise and daidzein synergistically mobilized and redistributed natural killer cells through upregulating the level of epinephrine and interleukin-6. Moreover, exercise combined with daidzein induces apoptosis in cancer cellsviaFas/FasL-initiated mitochondrial apoptosis signaling pathway. These results suggested that regular exercise combined with daidzein may explore a candidate way to prevent and treat the breast cancer.