Clinical impact of Candida spp. biofilm production in a cohort of patients with candidemia
Clinical impact of Candida spp. biofilm production in a cohort of patients with candidemia
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DOI：
10.1093/mmy/myx133
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发表时间：
2018-10-01
期刊：
影响因子：
2.9
通讯作者：
Colombo, Arnaldo Lopes
中科院分区：
文献类型：
--
作者：
Monfredini, Priscilla Morais;Remondi Souza, Ana Carolina;Colombo, Arnaldo Lopes
持续念珠菌症是指尽管接受了适当的治疗，念珠菌病患者的血液中仍继续分离出同一种念珠菌。尽管持续性念珠菌血症在临床上很重要，但研究只是表面上解决了这一现象背后的生物学条件。本研究的目的是评估念珠菌血液分离株的生物被膜形成能力与感染持久性之间的关系。对55株念珠菌进行了检测，并将其分为两组：(I)组I，包括7例持续念珠菌血症患者；(Ii)组II，包括18例非持续念珠菌血症患者。通过对核糖体DNA(RDNA)的内部转录间隔区(ITS)进行测序，在物种水平上鉴定了微生物。用结晶紫染色和共聚焦扫描激光显微镜(CSLM)对生物膜定量进行评价。分子检测证实了白色念珠菌(92%I组和94%II组)和都柏林假丝酵母菌分离株(8%I组和6%II组)的鉴定。55株细菌均能形成生物被膜，但持续组培养的白色念珠菌/都柏林念珠菌的生物被膜量较高(P
Persistent candidemia refers to the continued isolation of the same Candida species in the blood of a candidemic patient despite appropriate therapy. Despite the clinical importance of persistent candidemia, studies have superficially addressed the biological conditions behind this phenomenon. The aim of this studywas to evaluate the correlation between the biofilm-forming ability by Candida bloodstream isolates and the persistence of infection. A total of 55 isolates of Candida were tested and characterized in two groups: (i) group I, which included seven patients with persistent candidemia, and (ii) group II, which included 18 patients with nonpersistent candidemia. Microorganisms were identified at the species level by sequencing the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA). Biofilm quantification was evaluated by the crystal violet staining method and confocal scanning laser microscopy (CSLM). Molecular tests confirmed the identification of Candida albicans (92% group I and 94% group II) and Candida dubliniensis isolates (8% group I and 6% group II). All 55 isolates were able to form biofilms, but a higher biofilm mass was produced by C. albicans/C. dubliniensis strains cultured from the persistent group (P