Characterization of a putative maltodextrin-binding protein of Streptococcus pyogenes, SPs0871 and the development of a VHH inhibitor
Characterization of a putative maltodextrin-binding protein of Streptococcus pyogenes, SPs0871 and the development of a VHH inhibitor
复制标题
假定的化脓性链球菌麦芽糖糊精结合蛋白 SPs0871 的表征以及 VHH 抑制剂的开发
DOI：
10.1016/j.bbrc.2021.05.056
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发表时间：
2021
影响因子：
3.1
通讯作者：
Tsumoto K
中科院分区：
文献类型：
--
作者：
Yamawaki T;Nakakido M;Ujiie K;Aikawa C;Nakagawa I;Tsumoto K
化脓性链球菌引起广泛的人类感染。目前，抗生素是化脓性链球菌感染的主要治疗方法，但严重的抗生素耐药性需要替代治疗方案。为了开发一种新的治疗策略，我们的理化特性SPs 0871，一个假定的麦芽糖/麦芽糖糊精结合蛋白，被认为具有重要的作用，在侵袭性链球菌的发病机制。获得了与SPs 0871特异性结合的重链抗体的重链可变区，它是抗体的最小单位。尽管VHH完全抑制麦芽糖糊精与SPs 0871的结合，但这种抑制并不导致细菌的生长抑制。我们的研究结果为VHH作为抗链球菌治疗剂的发展提供了重要的见解。
Streptococcus pyogenescauses a wide range of human infections. Currently, antibiotics are the main treatment forS. pyogenesinfection, but serious anti-microbial resistance requires alternative treatment options. To develop a novel strategy for treatment, we physicochemically characterized SPs0871, a putative maltose/maltodextrin-binding protein that is thought to have important roles in the pathogenesis of invasive streptococci. We obtained a variable domain of heavy chain of heavy-chain antibody, the smallest unit of an antibody, which specifically binds to SPs0871. Although the VHH completely inhibited the binding of maltodextrins to SPs0871, the inhibition did not lead to growth suppression of the bacteria. Our results provide important insights for development of VHH as an anti-streptococcal therapeutic.