Fluorescent Glycopolymer Nanoparticles Based on Aggregation-Induced Emission Dyes: Preparation and Bioimaging Applications
Fluorescent Glycopolymer Nanoparticles Based on Aggregation-Induced Emission Dyes: Preparation and Bioimaging Applications
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基于聚集诱导发射染料的荧光糖聚合物纳米颗粒：制备和生物成像应用
DOI：
10.1002/macp.201400564
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发表时间：
2015
影响因子：
2.5
通讯作者：
Wei Yen
中科院分区：
文献类型：
--
作者：
Wang Ke;Zhang Xiaoyong;Zhang Xiqi;Yang Bin;Li Zhen;Zhang Qingsong;Huang Zengfang;Wei Yen
首次合成了基于聚集诱导发射（AIE）染料的荧光糖聚合物纳米颗粒（FGN），其表面具有丰富的碳水化合物基团。这首先通过AIE单体（PhE）和可再生的生物基单体衣康酸酐（ITA）之间的自由基聚合来进行，然后通过ITA和氨基葡萄糖盐酸盐之间的开环反应来进行，以获得其上具有大量糖基的糖基共聚物。所得两亲性糖基共聚物易于自组装成具有高吸水性的纳米颗粒，这是由于表面上覆盖的剩余羧基和糖基，其也可以进一步官能化。由于AIE染料位于纳米颗粒的核心，所获得的纳米颗粒表现出强烈的荧光发射。进一步研究了纳米颗粒的生物相容性评价和细胞摄取行为，以探索其潜在的生物医学应用;所展示的优异的生物相容性使其有望用于细胞成像。
Aggregation‐induced emission (AIE) dye‐based fluorescent glycopolymers nanoparticles (FGNs) are synthesized with abundant carbohydrate groups on the surface for the first time. This is carried out firstly by free radical polymerization between AIE monomer (PhE) and a renewable biobased monomer itaconic anhydride (ITA), then by the ring‐opening reaction between ITA and glucosamine hydrochloride, to obtain glycopolymers with plenty of glycosyl groups on them. The resulted amphiphilic glycopolymer is prone to self‐assemble into nanoparticles with high water dispersibility due to the surplus carboxyl groups and glycosyl groups covered on the surface, which can also be further functionalized. The obtained nanoparticles demonstrate strong fluorescence emission, owning to the AIE dyes in the core of nanoparticles. Biocompatibility evaluation and cell uptake behavior of the nanoparticles are further investigated to explore their potential biomedical applications; the demonstrated excellent biocompatibility makes them promising for cell imaging.