IL-10 and IFN-γ gene expression in chronic Chagas disease patients after in vitro stimulation with recombinant antigens of Trypanosoma cruzi
IL-10 and IFN-γ gene expression in chronic Chagas disease patients after in vitro stimulation with recombinant antigens of Trypanosoma cruzi
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DOI：
10.1016/j.cyto.2012.01.008
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发表时间：
2012-05-01
期刊：
影响因子：
3.8
通讯作者：
Gomes, Yara de Miranda
中科院分区：
文献类型：
--
作者：
de Melo, Adriene Siqueira;Barros de Lorena, Virginia Maria;Gomes, Yara de Miranda
与恰加斯病的其他几个方面一样，在慢性感染者中观察到的不同临床结果的机制尚未阐明。据信，宿主对寄生虫的免疫反应在病理学的发展中起着重要作用。因此，本研究的目的是评估用克氏锥虫重组抗原CRA（细胞质重复抗原）和FRA（鞭毛重复抗原）体外刺激外周血单核细胞（PBMC）后IL-10和IFN-γ基因表达谱与慢性恰加斯病的临床形式之间的关系。巴西伯南布哥州累西腓伯南布哥大学奥斯瓦尔多·克鲁斯大学医院查加斯病科选取了 20 名患有该病心脏型 (CARD) 的患者，其中 10 名患有轻度心脏型 (CARD 1)，10 名患有严重心脏型 (CARD 2)，以及 20 名患有不确定型 (IND) 的患者。将这些个体的PBMC在CRA或FRA存在下培养3天，并通过使用实时定量PCR检测其信使RNA来评估IL-10和IFN-γ基因表达。尽管研究的个体组之间没有观察到显着差异，但我们发现大多数 IND 患者表现出高水平的 IFN-gamma 基因表达，而大多数 CARD 1 患者表现出高水平的 IL-10。因此，这项研究的结果强调了炎症细胞因子在控制寄生虫复制的 IND 组患者中发挥的重要作用，以及抗炎细胞因子在确定疾病进展为有症状临床形式的易感性方面发挥的重要作用。 (C) 2012 Elsevier Ltd. 保留所有权利。
Along with several other aspects of Chagas disease, the mechanisms responsible for the different clinical outcomes observed in chronic infected individuals have not yet been clarified. It is believed that the host immune response to the parasite plays an important role in the development of the pathology. Therefore, the aim of this study was to evaluate the relationship between IL-10 and IFN-gamma gene expression profile, after in vitro stimulation of peripheral blood mononuclear cells (PBMC) with Trypanosoma cruzi recombinant antigens CRA (cytoplasmatic repetitive antigen) and FRA (flagellar repetitive antigen), and the clinical forms of chronic Chagas disease. Twenty patients with the cardiac form of the disease (CARD), of whom 10 had the mild cardiac form (CARD 1) and 10 the severe cardiac form (CARD 2), and 20 patients with the indeterminate form (IND), were selected at the Chagas Disease Unit of the Oswaldo Cruz University Hospital, University of Pernambuco, Recife, Pernambuco, Brazil. The PBMCs of these individuals were cultured in the presence of CRA or FRA for 3 days and IL-10 and IFN-gamma gene expression was evaluated by detection of its messenger RNA using Real Time Quantitative PCR. Although no significant difference was observed between the groups of individuals studied, we found that most patients with IND displayed high levels of IFN-gamma gene expression, while the majority of patients with CARD 1 presented high levels of IL-10. The results of this study thus highlight the important role that inflammatory cytokines play in patients with the IND group controlling for parasite replication, and that anti-inflammatory cytokines play in determining susceptibility to progression to symptomatic clinical forms of the disease. (C) 2012 Elsevier Ltd. All rights reserved.