3D analysis of human embryos and fetuses using digitized datasets from the Kyoto Collection.
3D analysis of human embryos and fetuses using digitized datasets from the Kyoto Collection.
复制标题
使用京都收藏的数字化数据集对人类胚胎和胎儿进行 3D 分析。
作者：
服部元史;Takakuwa T.
使用京都收集的数字化数据集对人类胚胎和早期胎儿期进行了三维（3D）分析，其中数字数据集在研究中发挥了主要作用。数据集包括使用1.5 T、2.35 T和7 T磁铁系统获得的磁共振成像（MRI）、相衬X射线计算机断层扫描（CT）和数字化的组织学序列切片。通过各种获取方法获得的涵盖大范围发育时期的大型、高分辨率数据集是研究的关键要素。数字数据有很大的优点，使我们能够进行各种分析。数字数据分析通过为分期人类胚胎的形态计量分析提供合适的平面，加快了使用精确和改进的方法进行形态学观察的速度。形态计量学数据对于定量评估和展示发育特征以及筛选异常样本有用，这可能提示先天性畸形的发病机制。形态计量学数据在“超声胚胎学”过程中对超声数据的比较也很有价值。解剖标志的三维坐标可作为分析有趣标志在发育过程中位置变化及其关系的有用工具。一些动态事件可以用三维坐标的微分增长来解释。此外，三维坐标可以用于数学分析和统计分析。我们研究中的3D分析可能有助于提供准确的形态学数据，包括与发育阶段相关的胚胎结构动力学，这是深入了解器官发生过程中发生的动态和复杂过程所必需的。中国生物医学工程学报，2018,31(1):96 - 96。©2018 Wiley期刊公司
Three‐dimensional (3D) analysis of the human embryonic and early‐fetal period has been performed using digitized datasets obtained from the Kyoto Collection, in which the digital datasets play a primary role in research. Datasets include magnetic resonance imaging (MRI) acquired with 1.5 T, 2.35 T, and 7 T magnet systems, phase‐contrast X‐ray computed tomography (CT), and digitized histological serial sections. Large, high‐resolution datasets covering a broad range of developmental periods obtained with various methods of acquisition are key elements for the studies. The digital data have gross merits that enabled us to develop various analysis. Digital data analysis accelerated the speed of morphological observations using precise and improved methods by providing a suitable plane for a morphometric analysis from staged human embryos. Morphometric data are useful for quantitatively evaluating and demonstrating the features of development and for screening abnormal samples, which may be suggestive in the pathogenesis of congenital malformations. Morphometric data are also valuable for comparing sonographic data in a process known as “sonoembryology.” The 3D coordinates of anatomical landmarks may be useful tools for analyzing the positional change of interesting landmarks and their relationships during development. Several dynamic events could be explained by differential growth using 3D coordinates. Moreover, 3D coordinates can be utilized in mathematical analysis as well as statistical analysis. The 3D analysis in our study may serve to provide accurate morphologic data, including the dynamics of embryonic structures related to developmental stages, which is required for insights into the dynamic and complex processes occurring during organogenesis. Anat Rec, 301:960–969, 2018. © 2018 Wiley Periodicals, Inc.