Feeding Administration of Daikenchuto Suppresses Colitis Induced by Naive CD4+ T Cell Transfer into SCID Mice
Feeding Administration of Daikenchuto Suppresses Colitis Induced by Naive CD4+ T Cell Transfer into SCID Mice
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DOI：
10.1007/s10620-012-2218-0
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发表时间：
2012-10-01
影响因子：
3.1
通讯作者：
Takayanagi, Ryoichi
中科院分区：
文献类型：
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作者：
Iwasa, Tsutomu;Ogino, Haruei;Takayanagi, Ryoichi
大研中藤是一种日本传统草药，通过改善胃肠蠕动和血液流动来抑制细菌移位。据报道，大研中托通过这些机制降低胃肠道炎症活动，我们分析了大研中托是否抑制实验性结肠炎和减少小鼠模型中的炎性细胞因子表达。通过将BALB/c小鼠的幼稚CD 4（+）T细胞转移到SCID小鼠中诱导结肠炎，并给予小鼠对照或含2.7%大研中托的饲料。我们研究了体重，临床症状，组织学变化，和Th 1-和Th 17-细胞因子的表达。使用实时RT-PCR分析细胞因子mRNA表达。流式细胞仪检测IL-17（+）和IFN-γ（+）CD 4（+）T细胞的比例。细胞因子mRNA的分析显示，在接受Daikenchuto的小鼠的结肠中，Th 17细胞因子显著减少。接受Daikenchuto的小鼠每结肠中IL-17（+）或IFN-γ（+）CD 4（+）T细胞的绝对数量低于接受对照饲料的小鼠，因为两组都接受了幼稚CD 4（+）T细胞以诱导结肠炎。Daikenchuto可能在治疗人类炎症性肠病方面显示出临床益处，需要进一步研究。
Daikenchuto, a traditional Japanese herbal medicine, suppresses bacterial translocation by improvement of gastrointestinal motility and blood flow. As Daikenchuto reportedly reduces gastrointestinal inflammatory activity by these mechanisms, we analyzed whether Daikenchuto suppresses experimental colitis and reduces inflammatory cytokine expression in a mouse model.Colitis was induced by transfer of naive CD4(+) T cells of BALB/c mice into SCID mice, and mice were given either control or 2.7 % Daikenchuto-containing feed. We investigated body weight, clinical symptoms, histological changes, and Th1- and Th17-cytokine expression. Cytokine mRNA expression was analyzed using real-time RT-PCR. The ratio of IL-17(+) and IFN-gamma(+) CD4(+) T cells were analyzed by flow cytometry.Daikenchuto delayed the development of colitis and significantly reduced the histological inflammation scores. Analyses of cytokine mRNA revealed that Th17 cytokines were significantly decreased in colons of mice that received Daikenchuto. Absolute numbers of IL-17(+) or IFN-gamma(+) CD4(+) T cells per colon were less in mice receiving Daikenchuto than in mice that received control feed, as both groups received naive CD4(+) T cells to induce colitis.We demonstrated that feeding administration of Daikenchuto suppresses colitis induced by naive CD4(+) T cell transfer into SCID mice. Daikenchuto may show clinical benefit in the treatment of human inflammatory bowel disease and further studies are warranted.