Bone and cartilage formation in an experimental model of distraction osteogenesis
Bone and cartilage formation in an experimental model of distraction osteogenesis
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DOI：
10.1097/00005131-199802000-00008
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发表时间：
1998-02-01
影响因子：
2.3
通讯作者：
Einhorn, TA
中科院分区：
文献类型：
--
作者：
Jazrawi, LM;Majeska, RJ;Einhorn, TA
目的：(A)建立一种可靠、可重复性的大鼠牵张成骨系统，为今后骨修复与再生的研究奠定基础。(B)描述和描述大鼠牵张成骨过程中的组织学事件，并确定软骨发育是否是该过程的正常组成部分。研究设计：特定物种的纵向时间研究。方法：20只大鼠制作股骨骨干中段截骨术，并应用专为牵引而设计的单侧外固定器。根据延长的时间和程度将动物分为五组。结果：牵张过程中，GAP组织表现为胶原束和成纤维细胞，沿牵张力方向纵向排列。这些胶原支架上似乎铺有编织骨，新形成的血管窦似乎是牵张间隙内骨形成的起点。所有主动牵张组均表现为牵张间隙内膜内骨化和周围软骨内骨化。然而，当牵张停止时，在间隙中观察到软骨内骨化。结论：牵张在牵张间隙中产生了抑制软骨形成的环境。然而，损伤的骨膜形成软骨是强制性的，似乎不受牵张的影响。牵张间隙内的骨形成发生在血管生成的地方。
Objectives: (a) To develop a reliable and reproducible system for distraction osteogenesis in the rat to establish a model for future investigations of bone repair and regeneration. (b) To describe and characterize the histological events in distraction osteogenesis in the rat and to determine whether cartilage development is a normal component of the process.Study Design: Species-specific, longitudinal time study.Methods: Twenty rats underwent production of a middiaphyseal femoral osteotomy and application of a monolateral external fixator specifically designed for distraction. Animals were divided into five groups based on the time and extent of lengthening. Results: During distraction, gap tissue showed collagen bundles and fibroblasts that were oriented longitudinally to the direction of the distraction force. Woven bone appeared to be laid down on these collagen scaffolds, and the newly formed vascular sinuses appeared to be the sites from which bone formation was initiated within the distraction gap. All groups undergoing active distraction showed intramembranous ossification in the distraction gap and endochondral ossification peripherally. However, when distraction was discontinued, endochondral ossification was observed in the gap.Conclusion: Distraction produces an environment in the distraction gap that suppresses the formation of cartilage. The formation of cartilage by injured periosteum, however, is obligatory and does not appear to be influenced by distraction. Bone formation within the distraction gap occurs where angiogenesis develops.