Effects of Interfacial Interactions on Electrocatalytic Activity of Cytochrome c Oxidase in Biomimetic Lipid Membranes on Gold Electrodes
Effects of Interfacial Interactions on Electrocatalytic Activity of Cytochrome c Oxidase in Biomimetic Lipid Membranes on Gold Electrodes
复制标题
界面相互作用对金电极仿生脂膜细胞色素c氧化酶电催化活性的影响
DOI：
10.1021/acs.jpclett.2c01765
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yagi Ichizo
中科院分区：
文献类型：
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作者：
Kato Masaru;Sano Ryoya;Yoshida Narumi;Iwafuji Masatoshi;Nishiyama Yoshito;Oka Sayuki;Shinzawa-Itoh Kyoko;Nishida Yuya;Shintani Yasunori;Yagi Ichizo
利用蛋白质膜电化学和表面增强红外吸收（SEIRA）技术研究了界面相互作用对含细胞色素氧化酶（CcO）的蛋白质束缚双层类脂膜（ptBLMs）对氧还原反应的电催化活性的影响.通过混合烷硫醇的自组装单分子膜（SAMs）将哺乳动物CcO固定在金电极上。蛋白质在电极上的取向是由SAM-CcO相互作用控制的，并且对细胞色素（cytc）结合至关重要。CcO-磷脂和CcO-cytc相互作用调节CcO的电催化活性，并且更密集地堆积的ptBLMs显示更高的电催化活性。我们的研究表明，光谱和电化学研究的ptBLMs可以提供洞察相对较弱的蛋白质-蛋白质和蛋白质-脂质相互作用的跨膜酶的酶活性的影响。
Effects of interfacial interactions on the electrocatalytic activity of protein-tethered bilayer lipid membranes (ptBLMs) containing cytochromecoxidase (CcO) for the oxygen reduction reaction are studied by using protein film electrochemistry and surface-enhanced infrared absorption (SEIRA) spectroscopy. Mammalian CcO was immobilized on a gold electrode via self-assembled monolayers (SAMs) of mixed alkanethiols. The protein orientation on the electrode is controlled by SAM–CcO interactions and is critical to the cytochromec(cytc) binding. The CcO–phospholipid and CcO–cytcinteractions modulate the electrocatalytic activity of CcO, and more densely packed ptBLMs show higher electrocatalytic activity. Our study indicates that spectroscopic and electrochemical studies of ptBLMs can provide insights into the effects of relatively weak protein–protein and protein–lipid interactions on the enzymatic activity of transmembrane enzymes.