Motility precedes egress of malaria parasites from oocysts
Motility precedes egress of malaria parasites from oocysts
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DOI：
10.7554/elife.19157
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发表时间：
2017-01-24
期刊：
影响因子：
7.7
通讯作者：
Frischknecht, Friedrich
中科院分区：
文献类型：
--
作者：
Klug, Dennis;Frischknecht, Friedrich
当受感染的按蚊叮咬时将疟原虫子孢子沉积在皮肤上时，就会传播疟疾。子孢子在蚊子中肠壁的卵囊内形成，并释放到血淋巴中，从血淋巴侵入唾液腺，随后传播到脊椎动物宿主。我们发现，血小板反应蛋白重复序列含有子孢子特异性蛋白，称为血小板反应蛋白相关蛋白 1 (TRP1)，对于卵囊排出和唾液腺侵袭非常重要，因此对于疟疾的传播也很重要。我们对卵囊中子孢子的释放进行了原位成像，在此之前有活跃的运动。缺乏TRP1的寄生虫无法在卵囊内迁移并且不会排出，这表明TRP1是卵囊内运动以及随后的子孢子排出和唾液腺入侵之前的事件的重要组成部分。
Malaria is transmitted when an infected Anopheles mosquito deposits Plasmodium sporozoites in the skin during a bite. Sporozoites are formed within oocysts at the mosquito midgut wall and are released into the hemolymph, from where they invade the salivary glands and are subsequently transmitted to the vertebrate host. We found that a thrombospondin-repeat containing sporozoite-specific protein named thrombospondin-releated protein 1 (TRP1) is important for oocyst egress and salivary gland invasion, and hence for the transmission of malaria. We imaged the release of sporozoites from oocysts in situ, which was preceded by active motility. Parasites lacking TRP1 failed to migrate within oocysts and did not egress, suggesting that TRP1 is a vital component of the events that precede intra-oocyst motility and subsequently sporozoite egress and salivary gland invasion.