Isolation and characterization of regulators involved in PHOT1-mediated inhibition of hypocotyl phototropism in Arabidopsis
Isolation and characterization of regulators involved in PHOT1-mediated inhibition of hypocotyl phototropism in Arabidopsis
复制标题
拟南芥中参与 PHOT1 介导的下胚轴向光性抑制的调节因子的分离和表征
DOI：
10.1515/biolog-2017-0070
复制
发表时间：
2017
期刊：
影响因子：
1.5
通讯作者：
Xiao Zhang
中科院分区：
文献类型：
--
作者：
Qing-Ping Zhao;Xiang Zhao;Zi-Yi Zhu;Xi-ning Guo;Nan-Nan Li;Xiao Zhang
摘要蓝光受体phot1和phot2在功能冗余中调节高强度蓝光诱导的下胚轴向光性。rpt 2单突变体的表型与失去向光性的双突变体phot1phot2相似，而rpt 2phot1双突变体表现出正常的向光性反应，表明phot1具有诱导和抑制下胚轴向光性的双重相反功能。然而，phot1介导的抑制机制仍有待阐明。本研究从EMS诱变的rpt 2基因中筛选出具有向光性的突变体，并克隆了可能位于phot1下游并参与调控phot1介导的抑制反应的基因P1SA 1（Phototropin1 Signaling Associated 1）。rpt 2 p1sa 1突变体对单侧HBL显示正常的向光性，而PHOT 1在rpt 2 p1sa 1中的过表达又使下胚轴的向光性丧失，表明P1SA 1可能是PHOT 1的等位基因。这些研究结果将为筛选和鉴定phot1介导的抑制反应相关基因开辟新的视角，并为植物减轻强光伤害的自我调节机制提供理论依据。
Abstract Blue light receptor phot1 and phot2 regulate hypocotyl phototropism induced by high intensity blue light (HBL) in functional redundancy. The phenotype of rpt2 single mutant is similar with double mutant phot1phot2 that lost phototropism, but rpt2phot1 double mutant shows normal phototropic response, which indicating that phot1 has dual opposite function with inducing and inhibiting hypocotyls phototropism. However, the mechanism of phot1-mediated inhibition remains to be elucidated. Here, we screened mutants with phototropism from EMS mutagenesis of rpt2 and cloned the gene P1SA1 (Phototropin1 Signaling Associated 1), which maybe locate downstream of phot1 and function in regulating phot1-mediated inhibitory response. The rpt2 p1sa1 mutant shows normal phototropism to unilateral HBL and overexpression of PHOT1 in rpt2 p1sa1 lost hypocotyl phototropism again, which indicated that P1SA1 may be the allelic of PHOT1. These results will open new perspectives about the screening and identification of genes involved in phot1-mediated inhibitory response, and provide theoretical basis for the self-regulation mechanism of plant to reduce high light damage.